Polyclonal-monoclonal elisa assay for detecting n-terminus pro-bnp

A quantitative method of measuring human NT-proBNP for predicting morbidity and mortality in a congestive heart failure patient, comprising:

• (a) providing isolated polyclonal antibodies specific for an amino acid sequence selected from the group consisting of amino acids 1-25 of SEQ ID NO:
• 1, amino acids 26-51 of SEQ ID NO:
• 1, and amino acids 52-76 of SEQ ID NO:
• 1;
• (b) contacting a bodily fluid sample from a patient to said isolated polyclonal antibodies;
• (c) providing proBNP monoclonal antibodies wherein said proBNP monoclonal antibodies are specific for the sequence of amino acid residues distinct from the specific sequence for the isolated polyclonal antibodies;
• (d) effecting an immunoreaction, wherein the immunoreaction is between a human NT-proBNP in the bodily fluid sample and the monoclonal antibodies;
• and
• (e) detecting said immunoreaction.

A kit for quantitative measurement of human NT-proBNP, comprising:

• isolated polyclonal antibodies specific for the sequence of amino acid selected from the group consisting of amino acids 1-25 of SEQ ID NO:
• 1, amino acids 26-51 of SEQ ID NO:
• 1, and amino acids 52-76 of SEQ ID NO. 1;
• isolated proBNP monoclonal antibodies, wherein said proBNP monoclonal antibodies are specific for the sequence of amino acid residues distinct from the isolated polyclonal antibodies, wherein the monoclonal antibodies are labeled with a detector;
• and
• a reporter capable of reacting with said detector to form a reaction product.