Microorganism having regulated expression of (r)-specific enoyl-coa hydratase gene and method for producing polyhydroxyalkanoate copolymer using same
AssigneeKaneka Corporation
InventorsHisashi ARIKAWA, Shunsuke SATO, Keiji MATSUMOTO
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Abstract
This invention relates to a microorganism that produces a polyhydroxyalkanoate (PHA) copolymer with a regulated monomer composition ratio and comprises a (R)-specific enoyl-CoA hydratase gene in the genome DNA, wherein a nucleotide sequence upstream of the (R)-specific enoyl-CoA hydratase gene comprises a modification consisting of a substitution(s), a deletion(s), an insertion(s), and/or an addition(s) of one or a plurality of nucleotides so that the expression of the (R)-specific enoyl-CoA hydratase gene is regulated, and to a method for producing a PHA copolymer using the microorganism.
Claims24
A microorganism comprising a (R)-specific enoyl-CoA hydratase gene in genome DNA, wherein a nucleotide sequence upstream of the (R)-specific enoyl-CoA hydratase gene comprises a modification that includes a substitution, a deletion, an insertion, and/or an addition of at least one nucleotide so that expression of the (R)-specific enoyl-CoA hydratase gene is regulated.
An expression regulatory DNA, which has a size of 30 bp to 900 bp and comprises a modified Cupriavidus necator phaC1 gene promoter, wherein the modified promoter comprises a nucleotide sequence selected from the group consisting of the nucleotide sequence of SEQ ID NOs:
- 55 to 59.
An expression regulatory DNA comprising a promoter comprising a nucleotide sequence selected from the group consisting of the nucleotide sequence of SEQ ID NOs:
- 47 to 50 and the nucleotide sequence of SEQ ID NOs:
- 51, 52, and 60 to 66.
Dependencies are inferred from the claim text.