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US 2017/0001196 A1Published application
Microfluidic based integrated sample analysis system
AssigneeBiomed Canada Corporation
InventorsGong Zhang, Francis Lin, Edward Stewart Richmond, Marshall Ring
Life of this patent
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Abstract
A portable microfluidic system capable of rapid diagnosis is described, which is able to analyze genetic, protein and cell composition of a sample in parallel for specific diseases from a relatively small sample. The method uses a single microfluidic chip integrated into a unique portable microfluidic platform and provides improved diagnostic accuracy, allows for frequent monitoring and is suitable for easy use in clinical settings.
Claims13
A sample analysis system comprising:
- a microfluidic chip comprising:
- a sample input for applying a sample to the chip;
- a separator connected to the sample input by a separation channel;
- one or more reaction channels, each reaction channel being connected to the separator and being of varying length,
- each said reaction channel being further connected to a respective outlet;
- and
- a reagent inlet;
- a thermoelectric semiconductor arranged to heat or cool one or more of said channels;
- an LED array arranged to emit radiation at a specific wavelength to the microfluidic chip;
- a detector arranged to detect fluorescence from said microfluidic chip;
- and
- a control unit arranged to control the valves and analyze results from the detector.
9 more elements
A microfluidic chip which comprises:
- a sample input for applying a sample to the microfluidic chip;
- a separator connected to the sample input by a separator channel, said separator separating a sample into cells and fluid;
- a cell analysis channel receiving the cells from the separator;
- and
- at least one analysis channel receiving the fluid from the separator.
2 more elements
A microfluidic chip which comprises:
- at least three layers:
- a top layer, a middle layer and a bottom layer;
- a sample input for applying a sample to the microfluidic chip, said sample input being at the top layer of the microfluidic chip;
- a separator connected to the sample input by a separator channel, said separator separating a sample into cells and fluid, said separator channel being at the top layer of the microfluidic chip and said separator being at the middle layer of the microfluidic chip;
- a cell analysis channel being at the bottom layer of the microfluidic chip and receiving the cells from the separator;
- and
- at least one analysis channel receiving the fluid from the separator.
4 more elements
A method of analyzing a sample comprising:
- providing a vacuum-sealed microfluidic chip which comprises:
- a sample input for applying a sample to the microfluidic chip;
- a separator connected to the sample input by a separator channel, said separator separating a sample into cells and fluid;
- a cell analysis channel receiving the cells from the separator;
- and
- at least one analysis channel receiving the fluid from the separator. and
- at least one reagent input;
- applying a sample comprising cells and fluid to the sample input;
- releasing the vacuum seal of the of the microfluidic chip such that the sample flows into the separator channel;
- separating the sample via the separator into cells and fluid;
- passing the cells to a cell analysis channel;
- separating the fluid into one or more analysis channels, said analysis channel being selected from the group consisting of a DNA analysis channel, an RNA analysis channel, an antibody detection channel, a protein analysis channel and combinations thereof;
- incubating the respective channels at suitable temperatures for a suitable period of time with a thermoelectric semiconductor to drive reactions to completion;
- emitting radiation over at least one suitable wavelength to the microfluidic chip viaan LED array;
- detecting florescence from the microfluidic chip with a detector;
- and
- analyzing results from the detector.
14 more elements
A method of analyzing a sample comprising:
- providing a vacuum-sealed microfluidic chip which comprises:
- at least three layers:
- a top layer, a middle layer and a bottom layer,
- a sample input for applying a sample to the microfluidic chip, said sample input being at the top layer of the microfluidic chip;
- a separator connected to the sample input by a separator channel, said separator separating a sample into cells and fluid, said separator channel being at the top layer of the microfluidic chip and said separator being at the middle layer of the microfluidic chip;
- a cell analysis channel being at the bottom layer of the microfluidic chip and receiving the cells from the separator;
- at least one analysis channel receiving the fluid from the separator;
- and
- at least one reagent input;
- applying a sample comprising cells and fluid to the sample input;
- releasing the vacuum seal of the of the microfluidic chip such that the sample flows into the separator channel;
- separating the sample via the separator into cells and fluid;
- passing the cells to a cell analysis channel;
- separating the fluid into one or more analysis channels, said analysis channel being selected from the group consisting of a DNA analysis channel, an RNA analysis channel, an antibody detection channel, a protein analysis channel and combinations thereof;
- incubating the respective channels at suitable temperatures for a suitable period of time with a thermoelectric semiconductor to drive reactions to completion;
- emitting radiation over at least one suitable wavelength to the microfluidic chip viaan LED array;
- detecting florescence from the microfluidic chip with a detector;
- and
- analyzing results from the detector.
16 more elements
Dependencies are inferred from the claim text.