Microfluidic device, system, and method

A micro-fluidic device for fluidic sample analysis, arranged to be positioned in a micro-fluidic controller comprising an external magnetic driver, comprising:

• a base structure;
• a flexible membrane;
• a micro-fluidic transition path limited by, at a bottom side, at least a portion of the base structure and by, at a top side, at least a portion of the flexible membrane, and extending between at least one inlet to be in communication with a first region and at least one outlet to be in communication with a second region;
• and
• wherein the microfluidic device is adapted such that, once in position in the micro-fluidic controller, the flexible membrane is placed in proximity to said external magnetic driver;
• wherein the micro-fluidic device further comprises:
• a plurality of magnetic particles (i) arranged to be put in contact and included thereafter in a fluid included in the microfluidic device or (ii) already included in the fluid and the micro-fluidic device further comprises this fluid;
• and
• wherein the micro-fluidic device is configured so that when the fluid containing the plurality of magnetic particles approaches or is within the micro-fluidic transition path and a magnetic force is applied by the external magnetic driver near the flexible membrane, at least a part of the magnetic particles are moved away from the base structure towards the flexible membrane, and wherein the flexible membrane is deflectable away from the base structure such that, when the flexible membrane is deflected, at least a part of the magnetic particles can be located, without the fluid or with a part of the fluid, beyond the in rest position of the flexible membrane;
• and
• wherein the micro-fluidic device is arranged such that the magnetic particles can move, without fluid or with only a part of the fluid, through the micro-fluidic transition path when an external magnetic driver applies a magnetic force in proximity to the micro-fluidic transition path.