Method for emulsifying a triepitope peptide with montanide and kits for performing the same

A method of obtaining a water in oil emulsion for administration of a polypeptide of sequence YLQVNSLQTVYLEYRQVPVYLEEITGYL (SEQ ID NO:

• 1) to a human subject, comprising:
• (i) obtaining an aqueous antigenic phase by dissolving lyophilized polypeptide of SEQ ID NO:
• 1 in pure sterile water;
• (ii) loading one volume of said aqueous antigenic phase and one volume of MONTANIDE ISA 51 into a device comprising two syringes linked by a connector;
• (iii) pre-emulsifying the formulation by performing at least three cycles of transfer of the whole formulation from one syringe to the other and then back to the first syringe, wherein each cycle lasts at least 6 seconds;
• and
• (iv) emulsifying the formulation by performing at least 20 additional transfer cycles, wherein each cycle lasts at most 2 seconds.