Back to results
US 2017/0000826 A1Published application
Methods and Devices for Preparation of Enriched Biological Fluids
AssigneeArthrex, Inc.
InventorsMelissa D. Tucker, Christopher Bare, Abigail Nabors, Robert Harrison
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to methods and apparatus for producing platelet rich plasma, bone marrow mononuclear cells, stromal vascular fraction from adipose tissue, and other concentrated or enriched biological fluids.
Claims19
A method of blood fractionation comprising:
- a) centrifuging whole blood in a first syringe having a top coupling element and a bottom coupling element, wherein the whole blood fractionates into a platelet poor plasma layer, a buffy layer, and an erythrocyte layer within the syringe;
- b) removing at least a portion of the platelet poor plasma layer from the top coupling element of the first syringe;
- c) attaching a bottom coupling element of a second syringe or second container to the top coupling element of the first syringe;
- d) drawing any of the platelet poor plasma layer, the buffy layer, and optionally part of the erythrocyte layer into the second syringe or second container;
- and
- e) centrifuging the second syringe or second container wherein the any of the platelet poor plasma layer, the buffy layer, and the erythrocyte layer fractionates into a platelet rich plasma layer and an erythrocyte layer.
3 more elements
A method of treating soft tissue damage comprising administering a composition comprising an autologous platelet rich plasma (PRP), wherein the PRP comprises about 8×106 to about 1×107 platelets/μL.
A method of bone marrow fractionation comprising:
- a) adding bone marrow to a first syringe having a top coupling element and a bottom coupling element;
- b) centrifuging the first syringe, wherein the bone marrow fractionates into a platelet poor plasma layer, a buffy layer, and an erythrocyte granulocyte layer within the first syringe;
- c) optionally removing at least a portion of the platelet poor plasma layer from the top coupling element of the first syringe;
- d) attaching a bottom coupling element of a second syringe or second container to the top coupling element of the first syringe;
- e) drawing any of the platelet poor plasma layer and the buffy layer into the second syringe or second container;
- and
- f) centrifuging the second syringe or second container wherein the platelet poor plasma layer and the buffy layer fractionates into a bone marrow mononuclear cell layer and a erythrocyte/granulocyte layer.
4 more elements
A method of adipose tissue fractionation comprising:
- a) centrifuging adipose tissue in a first syringe having a top coupling element and a bottom coupling element, wherein the adipose tissue fractionates into a lipid layer, a compressed adipose layer and an excess fluid layer within the first syringe;
- b) removing at least a portion of the lipid layer from the top coupling element of the first syringe;
- c) attaching a bottom coupling element of a second syringe to the top coupling element of the first syringe;
- d) drawing any of the lipid layer and the compressed adipose cell layer into the second syringe or second container;
- and
- e) centrifuging the second syringe wherein the lipid layer and the compressed adipose cell layer fractionates into an adipocyte layer, a lipid layer, and a stromal vascular fraction.
3 more elements
Dependencies are inferred from the claim text.