Compositions and Methods for Delivery of Gene Editing Tools Using Polymeric Vesicles
AssigneeVindico NanoBio Technology Inc.
InventorsP. Peter Ghoroghchian, Jivan Namdeo Yewle
Life of this patent
Estimating the term…
Abstract
A composition for genetic modification and a method of forming the composition, the composition may include a synthetic polymer vesicle, and a gene editing system encapsulated in the synthetic polymer vesicle. The gene editing system may include a protein component and a nucleic acid component configured to interact with a target sequence in a host cell genome.
Claims26
A composition for genetic modification, comprising:
- a synthetic polymer vesicle;
- and
- a gene editing system encapsulated in the synthetic polymer vesicle, the gene editing system comprising a nucleic acid component configured to interact with a target sequence in a host cell genome.
A method of modifying a host cell genome, the method comprising:
- encapsulating, in a polymersome, a gene editing system that comprises:
- a protein component;
- and
- a nucleic acid component configured to interact with a target nucleic acid sequence in the host cell;
- and
- delivering the encapsulated gene editing system to the host cell,
- wherein the polymersome is configured to selectively release the gene editing system in the host cell.
A method of manufacturing a suspension of an encapsulated gene editing composition, the method comprising:
- thermally blending a quantity of a block copolymer with a quantity of a low molecular weight polyethylene glycol (PEG) to create a PEG/polymer formulation;
- adding an aliquot of a solution of the gene editing composition to a sample containing the PEG/polymer formulation;
- and
- performing at least one dilution step such that polymersomes that are generated are progressively saturated with the gene editing composition,
- wherein the gene editing composition comprises:
- a protein component;
- and
- a nucleic acid component configured to interact with a target sequence in a host cell genome.
A kit, comprising:
- a pharmaceutical composition comprising a gene editing system encapsulated in a synthetic polymer vesicle, the gene editing system comprising:
- a protein component;
- and
- a nucleic acid component configured to interact with a target sequence in a host cell genome;
- and
- an implement for administering the pharmaceutical composition intravenously, via inhalation, topically, per rectum, per the vagina, transdermally, subcutaneously, intraperitoneally, intrathecally, intramuscularly, or orally.
Dependencies are inferred from the claim text.