Methods for biosynthesizing 1,3 butadiene
AssigneeInvista North America S.A.R.L.
InventorsPaul S. Pearlman, Changlin Chen, Adriana Leonora Botes, Alex Van Eck Conradie
Life of this patent
Estimating the term…
Abstract
This document describes biochemical pathways for producing butadiene by forming two vinyl groups in a butadiene synthesis substrate. These pathways described herein rely on enzymes such as mevalonate diphosphate decarboxylase, isoprene synthase, and dehydratases for the final enzymatic step.
Claims40
A method for the biosynthesis of butadiene, said method comprising forming two terminal vinyl groups in a butadiene synthesis substrate.
(canceled)
8-13. (canceled)
15-17. (canceled)
(canceled)
22-36. (canceled)
38-40. (canceled)
(canceled)
(canceled)
47-108. (canceled)
110-112. (canceled)
116-119. (canceled)
(canceled)
123-151. (canceled)
A non-naturally occurring biochemical network comprising at least one substrate of FIG. 1, FIG. 2, FIG. 3, FIG. 4, FIG. 5, FIG. 6, FIG. 7, FIG. 8, FIG. 9, FIG. 10 or FIG. 11, at least one exogenous nucleic acid encoding a polypeptide having the activity of at least one enzyme of FIG. 1, FIG. 2, FIG. 3, FIG. 4, FIG. 5, FIG. 6, FIG. 7, FIG. 8, FIG. 9, FIG. 10 or FIG. 11 and at least one product of FIG. 1, FIG. 2, FIG. 3, FIG. 4, FIG. 5, FIG. 6, FIG. 7, FIG. 8, FIG. 9, FIG. 10 or FIG. 11.
A non-naturally occurring composition, comprising at least one substrate of FIG. 1, FIG. 2, FIG. 3, FIG. 4, FIG. 5, FIG. 6, FIG. 7, FIG. 8, FIG. 9, FIG. 10 or FIG. 11, wherein said substrate is optionally bio-based, bio-derived or fermentation derived;
- at least one exogenous nucleic acid encoding a polypeptide having the activity of at least one enzyme of FIG. 1, FIG. 2, FIG. 3, FIG. 4, FIG. 5, FIG. 6, FIG. 7, FIG. 8, FIG. 9, FIG. 10 or FIG. 11;
- and at least one bio-based, bio-derived or fermentation derived product of FIG. 1, FIG. 2, FIG. 3, FIG. 4, FIG. 5, FIG. 6, FIG. 7, FIG. 8, FIG. 9, FIG. 10 or FIG. 11.
Dependencies are inferred from the claim text.