# US 2016/0355779 A1 · Method for Producing Fungus Structures > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2016/0355779 A1 - Document ID: US20160355779A1 - Status: Published application - Kind: A1 - Application number: 15/230,438 - Filed: 2016-08-07 - Published: 2016-12-08 - Earliest claimed: 2010-11-27 - Assignees: MycoWorks, Inc. - Inventors: Philip Ross - Web page: https://patentgrep.com/patent/US20160355779A1 ## CPC - C12N 1/22: Processes using, or culture media containing, cellulose or hydrolysates thereof - A01G 18/00: Cultivation of mushrooms - A01G 18/20: Culture media, e.g. compost - C12N 1/14: Fungi (culture of mushrooms A01G18/00; as new plants A01H15/00); Culture media therefor - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - A01G: HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING (picking of fruits, vegetables, hops or the like A01D46/00; propagating unicellular algae C12N1/12) ## Abstract A method for growing organically derived building materials in the form of a moldable substrate that can be engineered to serve a wide range of manufacturing and construction applications is presented. In particular, the embodiments consider a plurality of fungal molded shapes preferably grown from fungal inoculum and mechanically compressed at least once during the growing process as well as integration of structure support members to the fungal structure. The present invention provides a fungal substrate which could be molded, and easily and cheaply preprocessed to precise geometric specifications. The organically derived building materials also incorporate layers of structural reinforcements to improve load bearing and other structural capacities. ## Claims ### Claim 1 A method for growing organically derived building materials in the form of a moldable substrate to serve a wide range of manufacturing and construction applications, the method comprising the steps of: a) obtaining a lignocellulose based medium capable of supporting the growth of saprophytic fungi; b) mixing said lignocellulose based medium with water to reach a hydration level; c) inoculating said lignocellulose based medium with a fungal inoculum; d) allowing time for said inoculated lignocellulose based medium to become colonized to the extent that said inoculated lignocellulose based medium is transformed into a fungal mycelium without any secondary organisms displacing the process through infection; e) strictly regulating environmental conditions surrounding the lignocellulose based medium during said inoculation step and allowing step; f) adding a primary compressive pressure on the lignocellulose based medium of at least 100 PSI; g) reducing said primary compressive pressure; and h) drying said colonized fungal mycelium for a specific time period. ### Claim 2 (depends on 1) The method of claim 1 wherein said drying step renders the fungal inoculum biologically inert. ### Claim 3 (depends on 1) The method of claim 1 wherein said hydration level is between 33% and 66%. ### Claim 4 (depends on 1) The method of claim 1 wherein said drying step further comprises dehydrating the fungal molded shape such that water weight of said fungal molded shape is at most 15% of the total weight of said fungal molded shape. ### Claim 5 (depends on 1) The method of claim 1 wherein the fungal inoculum is a compressed form of mycelium fungi. ### Claim 6 (depends on 5) The method of claim 5 wherein the fungal inoculum is selected from the group consisting of: Ganoderma lucidem, Ganoderma tsugae, Ganoderma oregonense, Fomes fomentarius, Trametes versicolor and Piptoporous betulinus. ### Claim 7 (depends on 1) The method of claim 1 further comprising: applying stress pressure to said fungal mycelium such that cracks form in said fungal mycelium; and releasing said stress pressure such that said cracks are resealed by growth of said fungal mycelium. ### Claim 8 (depends on 1) The method of claim 1 wherein the lignocellulose based medium is combined with materials to change qualities and attributes of the fungal mycelium and the lignocellulose based medium, wherein the materials are selected from the group consisting of: silica, perlite, methyl cellulose, glycerin, and agarose. ### Claim 9 (depends on 1) The method of claim 1 wherein the fungal mycelium is combined with secondary materials to further create structural connections, mechanical reinforcements, and interfacings within and on the surface of the fungal mycelium. ### Claim 10 (depends on 1) The method of claim 1 further comprising pulverizing said fungal mycelium into a plurality of small pieces. ### Claim 11 (depends on 1) The method of claim 1 wherein said primary compressive pressure is applied through use of a roller. ### Claim 12 (depends on 11) The method of claim 11 further comprising pulverizing said fungal inoculum into a plurality of small pieces. ### Claim 13 (depends on 1) The method of claim 1 further comprising the steps of: applying a secondary compressive pressure of at least 100 PSI of pressure to said growing fungal inoculum, said secondary compressive pressure applied after termination of said primary compressive pressure; and releasing said secondary compressive pressure to said growing fungal inoculum. ### Claim 14 (depends on 13) The method of claim 13 further comprising applying a tertiary compressive pressure of at least 100 PSI to said growing fungal inoculum. ### Claim 15 (depends on 13) The method of claim 13 wherein at least one of either said primary compressive pressure or said secondary compressive pressure is of at least 500 PSI. ### Claim 16 (depends on 13) The method of claim 13 wherein at least one of either said primary compressive pressure or said secondary compressive pressure is of at least 2000 PSI. ### Claim 17 (depends on 13) The method of claim 13 wherein at least one compressive pressure is sufficient to cause saturated water within the growing fungal inoculum to be forced out, and then removing said at least one compressive pressure and allowing said growing fungal inoculum to naturally expand and absorb an agent. ### Claim 18 (depends on 17) The method of claim 17 wherein said agent is either a fluid or a gas. ### Claim 19 (depends on 1) The method of claim 1 further comprising pasteurizing said lignocellulose based medium for a specific time, said pasteurizing step terminating subsequent to the termination of said mixing step. ### Claim 20 (depends on 1) The method of claim 1 wherein said primary compressive pressure is of at least 500 PSI and applied for at least 72 hours.