# US 2016/0355774 A1 · Cell culturing device

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- Number: US 2016/0355774 A1
- Document ID: US20160355774A1
- Status: Published application
- Kind: A1
- Application number: 15/118,942
- Filed: 2015-02-17
- Published: 2016-12-08
- Earliest claimed: 2014-02-17
- Assignees: Asahi Kasei Kabushiki Kaisha; Tokyo Women's Medical University
- Applicants: Asahi Kasei Kabushiki Kaisha; Tokyo Women's Medical University
- Inventors: Kanako KONISHI; Ushio IWAMOTO; Masanori WADA; Katsuhisa MATSUURA; Tatsuya SHIMIZU; Teruo OKANO
- Web page: https://patentgrep.com/patent/US20160355774A1

## CPC

- C12M 27/02: {Stirrer or mobile mixing elements}
- C12M 23/08: {Flask, bottle or test tube}
- C12M 23/38: {Caps; Covers; Plugs; Pouring means}
- C12M 29/04: {Filters; Permeable or porous membranes or plates, e.g. dialysis}
- C12M 33/04: {by injection or suction, e.g. using pipettes, syringes, needles (pipettes in general B01L3/02)}
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}

## Abstract

A cell culturing device includes a culture tank, a shaft member at least partially disposed in the culture tank, a stirring mechanism supported by the shaft member, disposed in the culture tank, and a filter disposed in contact with the shaft member so as to suck the culture solution from the culture tank and/or to supply the culture solution to the culture tank. The shaft member is at least partially hollow, has openings to introduce the culture solution to the interior or to discharge the culture solution from the interior, and is unrotatable. Suction of the culture solution from the culture tank and/or supply of the culture solution to the tank is performed through the filter and the interior of the shaft member.

## Claims

### Claim 1

A cell culturing device comprising:
a culture tank configured to accommodate culture solution containing a cell;
a shaft member at least partially disposed in the culture tank;
stirring means supported by the shaft member and disposed in the culture tank, the stirring means having a stirring blade configured to be rotatable about the shaft member; and
a filter disposed in contact with the shaft member, the filter allowing the culture solution to pass through and not allowing the cell to pass through, wherein
the shaft member is at least partially hollow, has an opening to introduce the culture solution to interior of the shaft member or to discharge the culture solution from the interior, and is configured to be unrotatable,
the filter is located inner than a movable region of the stirring blade rotating about the shaft member and is disposed on the shaft member independently of the stirring blade, and
suction of the culture solution from the culture tank and/or supply of the culture solution to the culture tank is performed through the filter and the interior of the shaft member.

### Claim 2 (depends on 1)

The cell culturing device according to claim 1, wherein the shaft member is supported by a bottom of the culture tank.

### Claim 3 (depends on 2)

The cell culturing device according to claim 2, wherein
the shaft member has a lower end having the opening and has an upper end closed,
the shaft member has a side surface having an opening in communication with the interior, and
the filter is disposed on an outer surface of the shaft member so as to cover the opening at the side surface.

### Claim 4 (depends on 3)

The cell culturing device according to claim 3, wherein the filter has a tubular shape and extends on outside of the shaft member so as to cover the opening at the side surface.

### Claim 5 (depends on 2)

The cell culturing device according to claim 2, wherein
the shaft member has a tubular shape that has a lower end having the opening and that has an upper end having an opening in communication with the interior, and
the filter is disposed on the shaft member so as to cover the opening at the upper end.

### Claim 6 (depends on 5)

The cell culturing device according to claim 5, wherein the filter has a tubular shape with a base and extends on outside of the shaft member so as to cover the opening at the upper end.

### Claim 7 (depends on 1)

The cell culturing device according to claim 1, wherein the shaft member is supported by a lid of the culture tank.

### Claim 8 (depends on 7)

The cell culturing device according to claim 7, wherein
the shaft member has a tubular shape that has an upper end having the opening and that has a lower end having an opening in communication with the interior, and
the filter is disposed on the shaft member so as to cover the opening at the lower end.

### Claim 9 (depends on 8)

The cell culturing device according to claim 8, wherein the filter has a tubular shape with a base and extends on outside of the shaft member so as to cover the opening at the lower end.

### Claim 10 (depends on 7)

The cell culturing device according to claim 7, wherein
the shaft member has an upper end having the opening and has a lower end closed,
the shaft member has a side surface having an opening in communication with the interior, and
the filter is disposed on an outer surface of the shaft member so as to cover the opening at the side surface.

### Claim 11 (depends on 10)

The cell culturing device according to claim 10, wherein the filter has a tubular shape and extends on outside of the shaft member so as to cover the opening at the side surface.

### Claim 12 (depends on 1)

The cell culturing device according to any one of claims claim 1, wherein the filter is porous.