# US 2016/0355773 A1 · Cell culture container

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- Number: US 2016/0355773 A1
- Document ID: US20160355773A1
- Status: Published application
- Kind: A1
- Application number: 15/240,284
- Filed: 2016-08-18
- Published: 2016-12-08
- Earliest claimed: 2014-02-20
- Assignees: Tokyo Electron Limited
- Applicants: Tokyo Electron Limited
- Inventors: Tomoaki KURAKAZU; Yasuhiro OSHIMA; Yoshio KIMURA; Kenichi KAGAWA; Shinichi GOMI; Shigenori OZAKI
- Web page: https://patentgrep.com/patent/US20160355773A1

## CPC

- C12M 23/12: {Well or multiwell plates (C12M25/04 takes precedence)}
- C12M 23/04: {Flat or tray type, drawers (C12M23/10, C12M23/12, C12M23/16 take precedence)}
- C12M 23/20: {Material Coatings (immunocoatings C12M25/00)}
- C12M 23/24: {Gas permeable parts}
- C12M 25/00: {Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings}
- C12M 25/06: {Plates; Walls; Drawers; Multilayer plates}
- C12M 29/10: {Perfusion}
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}

## Abstract

A cell culture container characterized by being equipped with a container main body and a flat plate attached onto one surface of the container main body, wherein the container main body is equipped with an inflow port through which a liquid can flow into the container main body, a passage through which the liquid flowing into the container main body from the inflow port can pass, and an outflow port through which the liquid passing through the passage can flow out from the container main body, and wherein, on the bottom surface of the passage, multiple cell-seeding areas in which cells passing through the passage can be seeded are arranged side by side along the passage.

## Claims

### Claim 1

A cell culture container, comprising:
a container main body; and
a flat plate attached to one surface of the container main body,
wherein the container main body includes an inflow port through which a liquid flows into the container main body, a passage through which the liquid flowing into the container main body from the inflow port passes, and an outflow port through which the liquid passing through the passage flows out from the container main body, and
a plurality of cell-seeding areas, in which cells passing through the passage are seeded, is provided on a bottom surface of the passage and is arranged side by side along the passage,
wherein a diameter of the passage is from 2 mm to 4 mm.

### Claim 2 (depends on 1)

The container of claim 1, wherein a ratio of width and length of the passage is set such that the width:the length is equal to 3:60 to 1800.

### Claim 3 (depends on 1)

The container of claim 1, wherein the flat plate is fixedly supported by a tip region of a wall that defines the passage.

### Claim 4 (depends on 1)

The container of claim 1, wherein the cell-seeding areas are provided at regular intervals along the passage.

### Claim 5 (depends on 1)

The container of claim 1, wherein the passage includes a serpentine portion.

### Claim 6 (depends on 1)

The container of claim 1, wherein the passage includes a branching section in which the passage is branched into a plurality of passage components and a merging section in which the passage components are merged.

### Claim 7 (depends on 1)

The container of claim 1, wherein cavities are formed on the bottom surface of the passage in a concentric relationship with the cell-seeding areas.

### Claim 8 (depends on 7)

The container of claim 7, wherein the cavities have a pyramidal shape or a conical shape.

### Claim 9 (depends on 7)

The container of claim 7, wherein each of the cavities includes a flat bottom portion.

### Claim 10 (depends on 7)

The container of claim 7, wherein an extracellular matrix (ECM) is coated on a portion of the flat plate which faces the cavities.

### Claim 11 (depends on 9)

The container of claim 9, wherein an extracellular matrix (ECM) is coated inside the cavities and is not coated outside the cavities.

### Claim 12 (depends on 1)

The container of claim 1, wherein the bottom surface of the passage is flat and an extracellular matrix (ECM) is coated inside the cell-seeding areas and is not coated outside the cell-seeding areas.

### Claim 13 (depends on 1)

The container of claim 1, wherein an outer surface roughness of the cell-seeding areas is larger than an inner surface roughness of the cell-seeding areas.

### Claim 14 (depends on 1)

The container of claim 1, wherein the bottom surface of the passage and the flat plate have optical transparency.

### Claim 15 (depends on 1)

The container of claim 1, wherein the flat plate has gas permeability.