# US 2016/0355758 A1 · Hand dishwashing liquid detergent composition > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2016/0355758 A1 - Document ID: US20160355758A1 - Status: Published application - Kind: A1 - Application number: 15/161,466 - Filed: 2016-05-23 - Published: 2016-12-08 - Earliest claimed: 2015-06-04 - Assignees: The Procter & Gamble Company - Applicants: The Procter & Gamble Company - Inventors: Neil Joseph LANT - Web page: https://patentgrep.com/patent/US20160355758A1 ## CPC - C11D 3/38627: {containing lipase} - C11D 3/38681: {Chemically modified or immobilised enzymes} - C11D: DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL ## Abstract A hand dishwashing detergent composition having a surfactant system a lipase at least 0.05% by weight of the composition of at least one monovalent, divalent or trivalent cation or a mixture thereof. ## Claims ### Claim 1 A hand dishwashing liquid detergent composition comprising: i) a surfactant system; ii) at least one lipase wherein said lipase is a variant of a parent lipase, wherein said variant having lipase activity, at least 60% but less than 100% sequence identity with SEQ ID NO: 1, and comprises substitutions at positions corresponding to T231R+N233R and at least one or more of D96E, D111A, D254S, G163K, P256T, G91T and G38A of SEQ ID NO: 1; and iii) at least 0.05% by weight of the composition of a east one monovalent, divalent or trivalent cation or a mixture thereof. ### Claim 2 (depends on 1) The composition according to claim 1 wherein said variant further comprises substitutions at positions corresponding to D27R and/or N33Q of SEQ ID NO: 1. ### Claim 3 (depends on 1) The composition according to claim 1 wherein said variant comprises substitutions of SEQ ID NO: 1 selected from the group consisting of: a) D96E+T231R+N233R; b) N33Q+D96E+T231R+N233R; c) N33Q+D111A+T231R+N233R; d) N33Q+T231R+N233R+P256T; e) N33Q+G38A+G91T+G163K+T231R+N233R+D254S; f) N33Q+G38A+G91T+D96E+D111A+G163K+T231R+N233R+D254S+P256T; g) D27R+N33Q+G38A+D96E+D111A+G163K+T231R+N233R+D254S+P256T; h) D27R+N33Q+G38A+G91T+D96E+D111A+G163K+T231R+N233R+P256T; i) D27R+N33Q+G38A+G91T+D96E+D111A+G163K+T231R+N233R+D254S; j) D27R+G38A+G91T+D96E+D111A+G163K+T231R+N233R+D254S+P256T; k) D96E+T231R+N233R+D254S; l) T231R+N233R+D254S+P256T; m) G163K+T231R+N233R+D254S; n) D27R+N33Q+G38A+G91T+D96E+G163K+T231R+N233R+D254S+P256T; o) D27R+G91T+D96E+D111A+G163K+T231R+N233R+D254S+P256T; p) D96E+G163K+T231R+N233R+D254S; q) D27R+G163K+T231R+N233R+D254S; r) D27R+G38A+G91T+D96E+D111A+G163K+T231R+N233R+D254S; s) D27R+G38A+G91T+D96E+G163K+T231R+N233R+D254S+P256T; t) D27R+G38A+D96E+D111A+G163K+T231R+N233R+D254S+P256T: u) D27R+D96E+G163K+T231R+N233R+D254S; v) D27R+D96E+D111A+G163K+T231R+N233R+D254S+P256T; w) D27R+G38A+D96E+G163K+T231R+N233R+D254S+P256T; x) D111A+G163K+T231R+N233R+D254S+P256T; y) D111A+T231R+N233R; z) D111A+T231R+N233R+D254S+P256T; aa) D27R+D96E+D111A+G163K+T231R+N233R; bb) D27R+D96E+D111A+T231R+N233R; cc) D27R+G38A+D96E+D111A+G163K+T231R+N233R+D254S+P256T; dd) D27R+N33Q+G38A+D96E+D111A+T231R+N233R+D254S+P256T; ee) D27R+G38A+D96E+D111A+G163K+E210Q+T231R+N233R+D254S+P256T; ff) D27R+T231R+N233R+D254S+P256T; gg) D96E+D111A+G163K+T231R+N233R; hh) D96E+D111A+G163K+T231R+N233R+D254S+P256T; ii) D96E+D111A+G163K+T231R+N233R+P256T; jj) D96E+D111A+T231R+N233R; kk) D96E+D111A+T231R+N233R+D254S; ll) D96E+D111A+T231R+N233R+D254S+P256T; mm) D96E+D111A+T231R+N233R+P256T; nn) D96E+G163K+T231R+N233R+D254S+P256T; oo) D96E+T231R+N233R+D254S+P256T; pp) D96E+T231R+N233R+P256T; qq) G38A+D96E+D111A+T231R+N233R; rr) G91T+D96E+D111A+G163K+T231R+N233R+D254S+P256T; ss) G91T+D96E+D111A+T231R+N233R; tt) G91T+D96E+T231R+N233R; uu) G91T+T231R+N233R+D254S+P256T; vv) N33Q+D96E+D111A+G163K+T231R+N233R+D254S+P256T; ww) T231R+N233R+D254S+P256T; and xx) T231R+N233R+P256T. ### Claim 4 (depends on 1) The composition according to claim 1 wherein said composition comprises at least 0.35% by weight of the composition of said cation. ### Claim 5 (depends on 1) The composition according to claim 1 wherein said composition comprises from about 0.35 to about 4% by weight of the composition of said cation. ### Claim 6 (depends on 1) The composition according to claim 1 wherein said cation is selected from the inorganic or organic salts of alkali metals, alkaline earth metals of aluminum, iron, copper and zinc, and wherein said anion source is selected from halides, sulphates, sulphites, carbonates, bicarbonates, phosphates, nitrates, nitrites, phosphates, formates, acetates, propionates, citrates, malates, tartrates, succinates, oxalates, lactates, and mixtures thereof. ### Claim 7 (depends on 1) The composition according to claim 1 wherein the cation is selected from sodium chloride, calcium chloride, potassium chloride, sodium sulfate, potassium sulfate, sodium acetate, potassium acetate, sodium formate, potassium formate, and mixtures thereof. ### Claim 8 (depends on 1) The composition according to claim 1 having a pH of from about 4 to about 9 as measured in a 10% aqueous solution in distilled water at 20° C. ### Claim 9 (depends on 1) The composition according to claim 1 wherein said composition comprises water, and by weight of the composition from about 5 to about 15% of an anionic surfactant, from about 0.8 to about 3% of an amphoteric surfactant, from about 0.001-2% of a lipase, at least about 0.05% of a monovalent, divalent or trivalent cation, and from about 1 to about 3% of a corresponding salt. ### Claim 10 (depends on 1) The composition according to claim 1 wherein said composition further comprises an amphoteric and or zwitteronic surfactant. ### Claim 11 (depends on 1) The composition according to claim 1 wherein said composition further comprises anon-surfactant. ### Claim 12 (depends on 1) The composition according to claim 1 wherein said composition further comprises an amylase. ### Claim 13 (depends on 1) The composition according to claim 1 wherein said composition further comprises a protease. ### Claim 14 (depends on 1) The composition according to claim 1 wherein said composition comprises from about 0.001 to about 2% by weight of the composition of lipase. ### Claim 15 (depends on 1) A method of manually washing dishware comprising the steps of: delivering a composition according to claim 1 to a volume of water to form a wash liquor and immersing the dishware in the liquor. ### Claim 16 (depends on 1) A method of manually washing dishware comprising the steps of: delivering a composition according to claim 1 directly onto the dishware or onto a cleaning implement and using said cleaning implement to clean the dishware.