# US 2016/0355571 A1 · Libraries of genetic packages comprising novel hc cdr1, cdr2, and cdr3 and novel lc cdr1, cdr2, and cdr3 designs

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- Number: US 2016/0355571 A1
- Document ID: US20160355571A1
- Status: Published application
- Kind: A1
- Application number: 15/177,199
- Filed: 2016-06-08
- Published: 2016-12-08
- Earliest claimed: 2008-04-24
- Assignees: Dyax Corp.
- Applicants: Dyax Corp.
- Inventors: Robert Charles Ladner
- Web page: https://patentgrep.com/patent/US20160355571A1

## CPC

- C40B 40/08: Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
- C07K 16/005: {constructed by phage libraries}
- C40B 40/02: Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
- C07K 2317/565: Complementarity determining region [CDR]
- C40B: COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES (in silico combinatorial libraries of nucleic acids, proteins or peptides G16B35/00; in silico combinatorial chemistry G16C20/60)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

Provided are compositions and methods for preparing and identifying antibodies having CDR3s that vary in sequence and in length from very short to very long which in certain embodiments may bind to a carbohydrate moiety or the active site of an enzyme. Libraries coding for antibodies with the CDR3s are also provided. The libraries can be provided by modifying a pre-existing nucleic acid library.

## Claims

### Claim 1

A library of vectors or genetic packages that display, display and express, or comprise a member of a diverse family of human antibody related peptides, polypeptides and proteins and collectively display, display and express, or comprise at least a portion of the diversity of the antibody family, wherein the vectors or genetic packages comprise variegated DNA sequences that encode a heavy chain (HC) CDR3, wherein 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or 35 of the positions in the HC CDR3 of the family are varied among Tyr, Gly, Asp, Ser, and Arg residues.

### Claim 2 (depends on 1)

The library of claim 1, wherein about 50% of all the positions of the HC CDR3 are Tyr, Gly, Asp, Ser, or Arg.

### Claim 3 (depends on 1)

The library of claim 1, wherein an Arg residue is present in a filler region between V and D, between D and J, or between V and J.

### Claim at position 4

-5. (canceled)

### Claim 6

A library of vectors or genetic packages that display, display and express, or comprise a member of a diverse family of human antibody related peptides, polypeptides and proteins and collectively display, display and express, or comprise at least a portion of the diversity of the antibody family, wherein the vectors or genetic packages comprise variegated DNA sequences that encode a heavy chain (HC) CDR3, wherein more than about 20% of the HC CDR3 sequence consists of Tyr residues.

### Claim 7 (depends on 6)

The library of claim 6, wherein D regions and J stumps of the HC CDR3 consist of more than about 20% of Tyr residues.

### Claim 8

A library of vectors or genetic packages that display, display and express, or comprise a member of a diverse family of human antibody related peptides, polypeptides and proteins and collectively display, display and express, or comprise at least a portion of the diversity of the antibody family, wherein the vectors or genetic packages comprise variegated DNA sequences that encode a heavy chain (HC) CDR3, wherein less than about 20% of the HC CDR3 sequence consists of Tyr residues.

### Claim 9 (depends on 8)

The library of claim 8, wherein leadin or DJ filler positions of the HC CDR3 consist of less than about 20% of Tyr residues.

### Claim at position 9

-28. (canceled)