# US 2016/0355445 A1 · Agricultural microbial inoculant compositions and uses thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2016/0355445 A1 - Document ID: US20160355445A1 - Status: Published application - Kind: A1 - Application number: 15/170,813 - Filed: 2016-06-01 - Published: 2016-12-08 - Earliest claimed: 2015-06-02 - Assignees: Koch Agronomic Services, LLC - Applicants: Koch Agronomic Services, LLC - Inventors: Drew R. Bobeck; Cedric J. Pearce - Web page: https://patentgrep.com/patent/US20160355445A1 ## CPC - C05F 11/08: Organic fertilisers containing added bacterial cultures, mycelia or the like - A01N 63/22: Bacillus - A01N 63/23: B. thuringiensis - C05G 3/00: Mixtures of one or more fertilisers with additives not having a specially fertilising activity - C05G 3/90: for affecting the nitrification of ammonium compounds or urea in the soil - C05G 5/23: Solutions - Y02P 60/21: Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures - C05F: ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - C05G: MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS (organic fertilisers containing added bacterial cultures, mycelia, or the like C05F11/08; organic fertilisers containing plant vitamins or hormones C05F11/10); FERTILISERS CHARACTERISED BY THEIR FORM - Y02P: CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS ## Abstract The present disclosure provides novel agricultural microbial inoculant compositions for uses in promoting plant growth, plant productivity and/or soil quality. The novel microbial inoculant compositions comprise one or more microbial species, one or more urease inhibitors and/or one or more nitrification inhibitors. The present disclosure also provides fertilizer compositions comprising said microbial inoculant compositions. ## Claims ### Claim 1 An agricultural microbial inoculant composition comprising: i. at least one agriculturally beneficial Bacillus species selected from the group consisting of Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus thuringiensis, Bacillus pumilis, and any combination thereof; and ii. at least one organic solvent selected from the group consisting of propylene glycol (PG), N-methyl-2-pyrrolidone (NMP), triethylene glycol monobutyl ether, glycerol, dimethyl sulfoxide (DMSO), and any combination thereof. ### Claim 2 (depends on 1) The agricultural microbial inoculant composition of claim 1, wherein the agriculturally beneficial Bacillus species is selected from the group consisting of Bacillus licheniformis, Bacillus thuringiensis, and any combination thereof. ### Claim 3 (depends on 1) The agricultural microbial inoculant composition of claim 1, wherein the agriculturally beneficial Bacillus species is selected from the group of strains consisting of Bacillus amyloliquefaciens (ATCC 23842), Bacillus licheniformis (ATCC 14580), Bacillus licheniformis (B-642), Bacillus licheniformis (B-14368), Bacillus thuringiensis (ATCC 10792), Bacillus thuringiensis (HD-17), Bacillus thuringiensis (HD-1), Bacillus pumilis (NRS-272), and any combination thereof. ### Claim 4 (depends on 1) The agricultural microbial inoculant composition of claim 1, further comprising a urease inhibitor selected from the group consisting of N-(n-butyl)thiophosphoric triamide (NBPT), N-(n-butyl)phosphoric triamide, thiophosphoryl triamide, phenyl phosphorodiamidate, N-cyclohexyl phosphoric triamide, N-cyclohexyl thiophosphoric triamide, phosphoric triamide, hydroquinone, p-benzoquinone, hexamidocyclotriphosphazene, thiopyridines, thiopyrimidines, thiopyridine-N-oxides, N,N-dihalo-2-imidazolidinone, N-halo-2-oxazolidinone, N-(2-nitrophenyl)thiophosphoric triamide, N-(2-nitrophenyl)phosphoric triamide, and any combination thereof. ### Claim 5 (depends on 4) The agricultural microbial inoculant composition of claim 4, wherein the urease inhibitor is N-(n-butyl)thiophosphoric triamide (NBPT). ### Claim 6 (depends on 1) The agricultural microbial inoculant composition of claim 1, further comprising a nitrification inhibitor selected from the group consisting of 2-chloro-6-trichloromethylpyridine, 5-ethoxy-3-trichloromethyl-1,2,4-thiadiazol, dicyandiamide, 2-amino-4-chloro-6-methyl-pyrimidine, 1,3-benzothiazole-2-thiol, 4-amino-N-1,3-thiazol-2-ylbenzene sulfonamide, thiourea, guanidine, 3,4-dimethylpyrazole phosphate, 2,4-diamino-6-trichloromethyl-5-triazine, poly etherionophores, 4-amino-1,2,4-triazole, 3-mercapto-1,2,4-triazole, potassium azide, carbon bisulfide, sodium trithiocarbonate, ammonium dithiocarbamate, 2,3,-dihydro-2,2-dimethyl-7-benzofuranol methylcarbamate, N-(2,6-dimethylphenyl)-N-(methoxyacetyl)-alanine methyl ester, ammonium thiosulfate, 1-hydroxypyrazole, 3-methylpyrazole-1-carboxamide, 3-methylpyrazole, 3,5-dimethylpyrazole, 1,2,4-triazole, G77 Nitrification Inhibitor (CAS Registration No. 1373256-33-7), and any combination thereof. ### Claim 7 (depends on 6) The agricultural microbial inoculant composition of claim 6, wherein the nitrification inhibitor is dicyandiamide, G77 Nitrification Inhibitor (CAS Registration No. 1373256-33-7) or a combination thereof. ### Claim 8 (depends on 4) The agricultural microbial inoculant composition of claim 4, wherein the weight percentage of the urease inhibitor is in the range of 0.02-80% based on the total weight of the agricultural microbial inoculant composition. ### Claim 9 (depends on 8) The agricultural microbial inoculant composition of claim 8, wherein the weight percentage of the urease inhibitor is in the range of 10-60%. ### Claim 10 (depends on 1) The agricultural microbial inoculant composition of claim 1, wherein the agriculturally beneficial Bacillus species has at least 2 hours of viability from the time when said agriculturally beneficial Bacillus species contacts said organic solvent. ### Claim 11 (depends on 1) The agricultural microbial inoculant composition of claim 1, wherein the agriculturally beneficial Bacillus species concentration is in the range of 1.0×102 1.0×1012 spores/mL. ### Claim 12 (depends on 1) The agricultural microbial inoculant composition of claim 1, further comprising a dye. ### Claim 13 (depends on 4) The agricultural microbial inoculant composition of claim 4, comprising propylene glycol and N-methyl-2-pyrrolidone. ### Claim 14 (depends on 13) The agricultural microbial inoculant composition of claim 13, wherein the weight percentage of propylene glycol is in the range of 40-70% based on the total weight of the agricultural microbial inoculant composition, the weight percentage of N-methyl-2-pyrrolidone is in the range of 15-40% based on the total weight of the agricultural microbial inoculant composition, and the weight percentage of NBPT is in the range of 10-30% based on the total weight of the microbial inoculant composition. ### Claim 15 (depends on 4) The agricultural microbial inoculant composition of claim 4 comprising propylene glycol, N-methyl-2-pyrrolidone, and triethylene glycol monobutyl ether. ### Claim 16 (depends on 15) The agricultural microbial inoculant composition of claim 15, wherein the weight percentage of propylene glycol is in the range of 10-30% based on the total weight of the agricultural microbial inoculant composition, the weight percentage of N-methyl-2-pyrrolidone is in the range of 30-60% based on the total weight of the agricultural microbial inoculant composition, the weight percentage of triethylene glycol monobutyl ether is in the range of 1-5% based on the total weight of the agricultural microbial inoculant composition, and the weight percentage of NBPT is in the range of 15-40% based on the total weight of the agricultural microbial inoculant composition. ### Claim 17 An agricultural microbial inoculant kit comprising: Part A, wherein Part A comprises a urease inhibitor and at least one organic solvent, wherein said urease inhibitor is selected from the group consisted of N-(n-butyl)thiophosphoric triamide (NBPT), N-(n-butyl)phosphoric triamide, thiophosphoryl triamide, phenyl phosphorodiamidate, N-cyclohexyl phosphoric triamide, N-cyclohexyl thiophosphoric triamide, phosphoric triamide, hydroquinone, p-benzoquinone, hexamidocyclotriphosphazene,thiopyridines, thiopyrimidines, thiopyridine-N-oxides, N,N-dihalo-2-imidazolidinone, N-halo-2-oxazolidinone, N-(2-nitrophenyl)thiophosphoric triamide, N-(2-nitrophenyl)phosphoric triamide, and any combination thereof; and wherein said organic solvent is selected from the group consisting of propylene glycol (PG), N-methyl-2-pyrrolidone (NMP), triethylene glycol monobutyl ether, glycerol, dimethyl sulfoxide (DMSO), and any combination thereof; and Part B, wherein Part B comprises at least one Bacillus species selected from the group consisting of Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus thuringiensis, Bacillus pumilis, and any combination thereof. wherein each Part A and Part B is contained in a separate container. ### Claim 18 (depends on 17) The agricultural microbial inoculant kit of claim 17, wherein Part B comprises at least one Bacillus species selected from the group consisting of Bacillus licheniformis, Bacillus thuringiensis, and any combination thereof. ### Claim 19 (depends on 17) The agricultural microbial inoculant kit of claim 17, Part B comprises at least one agriculturally beneficial Bacillus strain selected from the group consisting of Bacillus amyloliquefaciens (ATCC 23842), Bacillus licheniformis (ATCC 14580), Bacillus licheniformis (B-642), Bacillus licheniformis (B-14368), Bacillus thuringiensis (ATCC 10792), Bacillus thuringiensis (HD-17), Bacillus thuringiensis (HD-1), Bacillus pumilis (NRS-272), and any combination thereof. ### Claim 20 (depends on 17) The agricultural microbial inoculant kit of claim 17, wherein the urease inhibitor is N-(n-butyl)thiophosphoric triamide (NBPT). ### Claim 21 (depends on 20) The agricultural microbial inoculant kit of claim 20, wherein Part A comprises propylene glycol and N-methyl-2-pyrrolidone. ### Claim 22 (depends on 21) The agricultural microbial inoculant kit of claim 21, wherein the weight percentage of propylene glycol is in the range of 40-70% based on the total weight Part A, the weight percentage of N-methyl-2-pyrrolidone is in the range of 15-40% based on the total weight of Part A, and the weight percentage of NBPT is in the range of 10-30% based on the total weight of Part A. ### Claim 23 (depends on 20) The agricultural microbial inoculant kit of claim 20, wherein Part A comprises propylene glycol, N-methyl-2-pyrrolidone, and triethylene glycol monobutyl ether. ### Claim 24 (depends on 23) The agricultural microbial inoculant kit of claim 23, wherein the weight percentage of propylene glycol is in the range of 10-30% based on the total weight of Part A, the weight percentage of N-methyl-2-pyrrolidone is in the range of 30-60% based on the total weight of Part A, the weight percentage of triethylene glycol monobutyl ether is in the range of 1-5% based on the total weight of Part A, and the weight percentage of NBPT is in the range of 15-40% based on the total weight of A. ### Claim 25 (depends on 17) The agricultural microbial inoculant kit of claim 17, wherein the agriculturally beneficial Bacillus species has at least 2 hours of viability from the time when said agriculturally beneficial Bacillus species in Part A contacts said organic solvent in Part B. ### Claim 26 (depends on 17) The agricultural microbial inoculant kit of claim 17, wherein the weight percentage of the urease inhibitor is in the range of 0.02-80% based on the total weight of Part A. ### Claim 27 (depends on 17) The agricultural microbial inoculant kit of claim 17, wherein the agriculturally beneficial Bacillus species concentration is in the range of 1.0×102 to 1.0×1012 spores/mL after Part A is mixed with Part B. ### Claim 28 (depends on 1) A method for promoting plant growth, plant productivity and/or soil quality, wherein the method comprises applying an effective amount of the agricultural microbial inoculant composition according to claim 1 to a plant, plant part, plant seed or soil. ### Claim 29 (depends on 1) A fertilizer composition comprising the agricultural microbial inoculant composition according to claim 1.