# US 2016/0354515 A1 · Scaffold for Tissue Growth and Repair

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- Number: US 2016/0354515 A1
- Document ID: US20160354515A1
- Status: Published application
- Kind: A1
- Application number: 15/242,711
- Filed: 2016-08-22
- Published: 2016-12-08
- Earliest claimed: 2009-03-12
- Assignees: New Jersey Institute of Technology
- Applicants: New Jersey Institute of Technology
- Inventors: Treena Arinzeh; George Collins; Yee-Shuan Lee
- Web page: https://patentgrep.com/patent/US20160354515A1

## CPC

- A61L 27/16: obtained by reactions only involving carbon-to-carbon unsaturated bonds
- A61L 27/3834: {Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells}
- A61L 27/54: Biologically active materials, e.g. therapeutic substances {(A61L27/227 takes precedence)}
- A61P 19/04: for non-specific disorders of the connective tissue
- A61P 19/08: for bone diseases, e.g. rachitism, Paget's disease
- C12N 5/0068: {General culture methods using substrates (for specific animal cell type C12N5/06)}
- C12N 5/0618: {Cells of the nervous system}
- C12N 5/0668: {Mesenchymal stem cells from other natural sources}
- A61L 2300/414: Growth factors
- A61L 2400/12: Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
- A61L 2430/02: for reconstruction of bones; weight-bearing implants
- A61L 2430/06: for cartilage reconstruction, e.g. meniscus
- C12N 2529/00: Culture process characterised by the use of electromagnetic stimulation
- C12N 2533/30: Synthetic polymers
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Provided is an electroactive structure and method for growing isolated differentiable cells comprising a three dimensional matrix of fibers formed of a biocompatible synthetic piezoelectric polymeric material, wherein the matrix of fibers is seeded with the isolated differentiable cells and forms a supporting scaffold for growing the isolated differentiable cells, and wherein the matrix of fibers stimulates differentiation of the isolated differentiable cells into a mature cell phenotype on the structure.

## Claims

### Claim 1

An electroactive structure for growing and differentiating a differentiable cell comprising a three dimensional matrix of electrospun biocompatible synthetic piezoelectric polymer fibers formed by electro spinning the polymer at a high electric potential, wherein the fiber matrix forms a scaffold for supporting cell growth and differentiation; and wherein the scaffold conditions are sufficient to induce differentiation of a mesenchymal stem cell into either an osteogenic or chondrogenic phenotype.

### Claim 2 (depends on 1)

The electroactive structure according to claim 1, wherein the biocompatible synthetic piezoelectric polymer is a homopolymer, a copolymer or combination thereof.

### Claim 3 (depends on 2)

The electroactive structure according to claim 2, wherein the homopolymer is a polyvinylidene fluoride (PVDF), or a trifluoroethylene polymer.

### Claim 4 (depends on 2)

The electroactive structure according to claim 2, wherein the copolymer is a poly(vinylidene fluoride trifluoroethylene) (PVDF-TrFE) copolymer.

### Claim 5 (depends on 3)

The electroactive structure according to claim 3, wherein the biocompatible synthetic piezoelectric homopolymer fibers are annealed.

### Claim 6 (depends on 4)

The electroactive structure according to claim 4, wherein the biocompatible synthetic piezoelectric copolymer fibers are annealed.

### Claim 7 (depends on 6)

The electroactive structure of claim 6, wherein the matrix of fibers further comprises a growth factor capable of further promoting the differentiation of the mesenchymal stem cell into a osteogenic or chondrogenic phenotype.

### Claim 8 (depends on 7)

The electroactive structure of claim 7, wherein the growth factor is associated with the matrix of fibers through at least one of a covalent interaction, a non-covalent interaction or a combination of both.

### Claim 9 (depends on 1)

The electroactive structure according to claim 1, wherein the matrix fibers is a non-woven mesh of nanofibers, microfibers or a combination of both.

### Claim 10 (depends on 1)

The electroactive structure according to claim 1, wherein matrix fibers are arranged in the matrix randomly, substantially aligned or a combination of both.

### Claim 11 (depends on 5)

The electroactive structure according to claim 5, wherein the fibers are thermally or chemically annealed, and wherein after annealing the fibers demonstrate enhanced piezoelectric characteristics, crystal organization or a combination of both.

### Claim 12 (depends on 6)

The electroactive structure according to claim 6, wherein the fibers are thermally or chemically annealed, and wherein after annealing the fibers demonstrate enhanced piezoelectric characteristics, crystallization, crystal structure or a combination thereof.

### Claim 13 (depends on 1)

The electroactive structure according to claim 1, wherein the osteogenic or chondrogenic phenotype is demonstrated by at least one of increased collagen expression, growth or a combination thereof.

### Claim 14 (depends on 1)

The electroactive structure according to claim 1, wherein the high electric potential is a voltage of at least between 15 to 30 kV.

### Claim 15 (depends on 1)

The electroactive structure according to claim 1, wherein the high electric potential is 25 kV.

### Claim 16

An implantable electroactive scaffold produced according to the steps comprising:
(a) electropsinning a biocompatible synthetic piezoelectric polymeric material at at least 25 kV to form a three-dimensional matrix of piezoelectric fibers, wherein the fibers have an average fiber diameter of from about 500 nm to about 5 μm;
(b) annealing the electrospun piezoelectric polymeric fibers; and
(c) seeding the annealed three-dimensional matrix of piezoelectric fibers with a mesenchymal stem cell under conditions sufficient to induce differentiation of a mesenchymal stem cell into either an osteogenic or chondrogenic phenotype.

### Claim 17 (depends on 16)

The implantable electroactive scaffold of claim 16, wherein the biocompatible synthetic piezoelectric polymeric material is at least one of a homopolymer, a copolymer or a combination of both.

### Claim 18 (depends on 17)

The implantable electroactive scaffold of claim 17, wherein the homopolymer is a polyvinylidene fluoride (PVDF), or a trifluoroethylene polymer.

### Claim 19 (depends on 17)

The implantable electroactive scaffold of claim 17, wherein the copolymer is a poly(vinylidene fluoride trifluoroethylene) (PVDF-TrFE) copolymer.

### Claim 20 (depends on 17)

The implantable electroactive scaffold of claim 17, further comprising the step of shaping the scaffold using a presss or mold.