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US 2016/0340728 A1Published application
Single-cell nucleic acid analysis
AssigneeFluidigm Corporation
InventorsAmy Hamilton, Min Lin, Alain Mir, Martin Pieprzyk
Life of this patent
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Abstract
The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.
Claims3
A method for genotyping a single cell, said method comprising:
- (a) performing whole genome amplification of the genome of a single cell to produce an amplified genome;
- (b) preamplifying the amplified genome to produce a preamplification reaction mixture comprising one or more amplicons specific for one or more target nucleic acids;
- and
- (c) amplifying and detecting the one or more amplicons.
1 more element
A method for analyzing RNA from a single cell, said method comprising:
- (a) preparing DNA from RNA from a single cell;
- (b) preamplifying the DNA to produce a preamplification reaction mixture comprising one or more amplicons specific for one or more target nucleic acids;
- and
- (c) amplifying and detecting the one or more amplicons.
1 more element
3-32. (canceled)
Dependencies are inferred from the claim text.