Use of peptidylglycine alpha-amidating monooxigenase (pam) for c-terminal amidation

A method for the recombinant production of a C-terminally amidated polypeptide characterized in that both the polypeptide and human peptidylglycine alpha-amidating monooxigenase (PAM) are recombinantly co-expressed in a mammalian cell.

The method according to any of the preceding claims, characterized in that the human peptidylglycine alpha-amidating monooxigenase (PAM) is a PAM 3 (SEQ ID NO:

• 02).

The method according to any of the preceding claims, characterized in that the mammalian cell comprises a first nucleic acid encoding the polypeptide and a second nucleic acid encoding the PAM.

The method according to any of the preceding claims, characterized in that the polypeptide is fused to the C-Terminus of an antibody heavy chain or the Fc region thereof.

The method according any of the preceding claims, characterized in that the polypeptide is Neurokinin, Allatostatin, Lem-KI, TRH, Red Pigment Concentrating Hormone, Calcitonin, CRF, LHRH, Leucopyrokinin, Gastrin I, Pigment Dispersing Hormone, Dermorphin, Oxytocin, Substance P, NPY, FMRFamide, Bombesin, Amylin, [Arg8]Vasopressin, BId-GrTH, Calcitonin, Cam-HrTH-II, Gastrin Releasing Peptide, Neuromedin B, Pancreastatin, Conotoxin M1, Secretin, GHRF, Melittin, Sarcotoxin 1A, VIP, α-MSH or MIF-1.

The method according any of the preceding claims, characterized in that the polypeptide is peptide YY (PYY 3-36) of SEQ ID NO:

• 05.

Use of a human peptidylglycine alpha-amidating monooxigenase (PAM) for the recombinant production of a C-terminally amidated polypeptide, characterized in that both the polypeptide and the human PAM are recombinantly co-expressed in a mammalian cell.