US 2016/0333331 A1 · IMPROVED ENZYME VARIANTS

Abstract

The invention relates to a variant polypeptide having lactase activity. The invention also relates to a nucleic acid sequence encoding such a variant polypeptide, to a nucleic acid construct comprising said nucleic acid sequence, to a recombinant expression vector comprising said nucleic acid construct and to a recombinant host cell comprising said expression vector. Further, the invention relates to a method for producing a lactase variant via use of such a host cell. Also, the invention relates to a method of producing a lactase polypeptide variant. The invention further relates to a composition comprising a lactase variant, to use of such a lactase variant or to the use of a lactase variant-containing composition in the preparation of a dairy product, to a process for the production of a dairy product and to the resulting dairy product.

Claims32

Dependent claims

A variant polypeptide having lactase activity, wherein the variant has an amino acid sequence which, when aligned with the lactase comprising the sequence set out in SEQ ID NO:

2, comprises at least one substitution of an amino acid residue corresponding to any of amino acids
233, 257, 258, 263, 274, 284, 297, 415, 440, 483, 619, 621, 622, 633, 862 or 1004 said positions being defined with reference to SEQ ID NO:
2 and wherein the variant has one or more altered properties as compared with a reference polypeptide having lactase activity and wherein said variant has at least 80% sequence identity with SEQ ID NO:2.

Dependencies are inferred from the claim text.

Improved enzyme variants

Life of this patent

Improved enzyme variants

Life of this patent

Abstract

Claims32