Biguanide Derivative, A Preparation Method Thereof, And A Pharmaceutical Composition Containing The Biguanide Derivative As An Active Ingredient

A method of activating AMPKα (5′-AMP-activated protein kinase alpha) in cells, comprising:

• administering a therapeutically effective amount of a compound of Formula 1 below or a pharmaceutically acceptable salt to a subject in need thereof:
• wherein R1, R2, R3 and R4 are independently hydrogen or a non-hydrogen substituent selected from the group consisting of C1-12 alkyl unsubstituted or substituted with at least one non-hydrogen substituent selected from the group consisting of C3-10 cycloalkyl, C5-12 aryl, C5-12 heteroaryl, hydroxyl, halogen and C1-4 alkoxycarbonyl;
• C3-10 cycloalkyl;
• C1-12 alkoxy;
• C5-12 aryl;
• C5-12 heteroaryl;
• hydroxyl;
• and halogen, and
• the aryl and heteroaryl are unsubstituted or substituted with at least one non-hydrogen substituent selected from the group consisting of C1-4 alkyl, C1-4 alkoxy, hydroxyl and halogen.

A method of treating a disease selected from the group consisting of diabetes mellitus, obesity, hyperlipidemia, hypercholesterolemia, fatty liver, coronary artery disease, osteoporosis, polycystic ovarian syndrome, metabolic syndrome, cancer, muscle pain, myocyte damage and rhabdomyolysis, comprising:

• administering a therapeutically effective amount of a compound of Formula 1 below or a pharmaceutically acceptable salt to a subject in need thereof:
• wherein R1, R2, R3 and R4 are independently hydrogen or a non-hydrogen substituent selected from the group consisting of C1-12 alkyl unsubstituted or substituted with at least one non-hydrogen substituent selected from the group consisting of C3-10 cycloalkyl, C5-12 aryl, C5-12 heteroaryl, hydroxyl, halogen and C1-4 alkoxycarbonyl;
• C3-10 cycloalkyl;
• C1-12 alkoxy;
• C5-12 aryl;
• C5-12 heteroaryl;
• hydroxyl;
• and halogen, and
• the aryl and heteroaryl are unsubstituted or substituted with at least one non-hydrogen substituent selected from the group consisting of C1-4 alkyl, C1-4 alkoxy, hydroxyl and halogen.