Mutant polypeptides and uses thereof
AssigneeINVISTA S.a.r.l.
InventorsAdriana L. Botes, Nadia Kadi, Mihai Luchian Azoitei, Yih-En A. Ban, Daniela Grabs-Röthlisberger, Alexander Pisarchik, Alexandre Zanghellini, Eric Althoff
Life of this patent
Estimating the term…
Abstract
The present disclosure provides novel polypeptides with 3-buten-2-ol dehydratase activity, polypeptides with catalytic activity in the conversion of 3-methyl-3-buten-2-ol to isoprene, and crystal structure data for one of such polypeptides. Methods of making and using the polypeptides and their related crystal structure data are also provided.
Claims10
-8. (canceled)
A composition comprising a rubber product or a plastic product polymerized from 1,3-butadiene produced in the presence of a polypeptide comprising the amino acid sequence of SEQ ID NO:462, wherein SEQ ID NO:462 is a triple mutant of SEQ ID NO:1 carrying the following three mutations:
- F382W, L328V, and I187M;
- or a signal-peptide-less version thereof (SEQ ID NO:460).
A composition comprising a rubber product or a plastic product polymerized from isoprene produced in the presence a polypeptide comprising the amino acid sequence of SEQ ID NO:462, wherein SEQ ID NO:462 is a triple mutant of SEQ ID NO:1 carrying the following three mutations:
- F382W, L328V, and I187M;
- or a signal-peptide-less version thereof (SEQ ID NO:460).
1,3-butadiene produced by the dehydration of 3-buten-2-ol to 1,3-butadiene in the presence of a polypeptide comprising the amino acid sequence of SEQ ID NO:462, wherein SEQ ID NO:462 is a triple mutant of SEQ ID NO:1 carrying the following three mutations:
- F382W, L328V, and I187M;
- or a signal-peptide-less version thereof (SEQ ID NO:460).
Isoprene produced by the dehydration of 3-methyl-3-buten-2-ol to isoprene in the presence of a polypeptide comprising the amino acid sequence of SEQ ID NO:462, wherein SEQ ID NO:462 is a triple mutant of SEQ ID NO:1 carrying the following three mutations:
- F382W, L328V, and I187M;
- or a signal-peptide-less version thereof (SEQ ID NO:460).
-20. (canceled)
Dependencies are inferred from the claim text.