Nucleic acids encoding plant glutamine phenylpyruvate transaminase (gpt) and uses thereof
AssigneesLos Alamos National Security, LLC, University of Maine System Board of Trustees
InventorsPat J Unkefer, Penelope S Anderson, Thomas J Knight
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Abstract
Glutamine phenylpyruvate transaminase (GPT) proteins, nucleic acid molecules encoding GPT proteins, and uses thereof are disclosed. Provided herein are various GPT proteins and GPT gene coding sequences isolated from a number of plant species. As disclosed herein, GPT proteins share remarkable structural similarity within plant species, and are active in catalyzing the synthesis of 2-hydroxy-5-oxoproline (2-oxoglutaramate), a powerful signal metabolite which regulates the function of a large number of genes involved in the photosynthesis apparatus, carbon fixation and nitrogen metabolism.
Claims28
-20. (canceled)
A nucleic acid construct comprising a heterologous plant promoter operably linked to an isolated polynucleotide encoding a glutamine phenylpyruvate transaminase (GPT) polypeptide having GPT catalytic activity.
A plant organ, embryo or seed comprising the nucleic acid construct according to claim 1, wherein the plant organ, embryo or seed expresses the polynucleotide.
Dependencies are inferred from the claim text.