Variants of cellobiohydrolases
AssigneeDanisco US Inc.
InventorsRichard R. BOTT, Maria FOUKARAKI, Ronaldus Wilhelmus HOMMES, Thijs KAPER, Bradley R. KELEMEN, Slavko KRALJ, Igor NIKOLAEV, Mats SANDGREN, Johannes Franciscus Thomas VAN LIESHOUT, SANDER VAN STIGT THANS
Life of this patent
Estimating the term…
Abstract
Disclosed are a number of homologs and variants of Hypocrea jecorina Cel7A (formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted and/or deleted.
Claims22
(canceled)
An isolated variant of a parent cellobiohydrolase (CBH) enzyme, wherein said variant has cellulase activity, has at least 80% sequence identity to SEQ ID NO:3, and wherein said variant comprises an amino acid substitution selected from the group consisting of:
- Y247D, N49P, T246V, N200G, and combinations thereof, wherein the position of each amino acid substitution corresponds to SEQ ID NO:3.
12-15. (canceled)
20-23. (canceled)
A method for hydrolyzing a cellulosic substrate, comprising:
- contacting said substrate with an isolated variant of a parent cellobiohydrolase (CBH) enzyme, wherein said variant has cellulase activity, has at least 80% sequence identity to SEQ ID NO:3, and wherein said variant comprises an amino acid substitution selected from the group consisting of:
- Y247D, N49P, T246V, N200G, and combinations thereof, wherein the position of each amino acid substitution corresponds to SEQ ID NO:3.
26-33. (canceled)
Dependencies are inferred from the claim text.