Agarooligosaccharide hydrolase and method for producing 3,6-anhydro-l-galactose and galactose from agarose by using same
AssigneeKorea University Research and Business Foundation
InventorsKyoung Heon Kim, In-Geol Choi, Chan Hyoung Lee, Hee Taek Kim, Eun-Ju Yun
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Abstract
The present invention relates to agarooligosaccharide hydrolase and a method for producing 3,6-anhydro-L-galactose and galactose from agarose by using the same. More specifically, the production yield of 3,6-anhydro-L-galactose and galactose from agarose, that is, the saccharification yield, is improved by using β-agarooligosaccharide hydrolase having an agarotriose hydrolytic activity.
Claims20
A composition for agarose saccharification comprising:
- an agarase;
- a β-agarooligosaccharide hydrolase having hydrolytic activity for agarotriose and represented by an amino acid sequence of SEQ ID NO:1;
- and
- an α-neoagarobiose hydrolase.
A composition for producing galactose comprising a β-agarooligosaccharide hydrolase having hydrolytic activity for agarotriose, agaropentaose, or agaroheptaose and represented by an amino acid sequence of SEQ ID NO:1.
Dependencies are inferred from the claim text.