# US 2016/0258971 A1 · Improved device and method for reactions between a solid and a liquid phase > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2016/0258971 A1 - Document ID: US20160258971A1 - Status: Published application - Kind: A1 - Application number: 15/032,193 - Filed: 2014-10-27 - Published: 2016-09-08 - Earliest claimed: 2013-10-28 - Assignees: EUROIMMUN Medizinische Labordiagnostika AG - Applicants: Euroimmun Medizinische Labordiagnostika AG - Inventors: Winfried STOECKER - Web page: https://patentgrep.com/patent/US20160258971A1 ## CPC - G01N 35/00029: {provided with flat sample substrates, e.g. slides (G01N35/028 takes precedence)} - B01L 3/502: {with fluid transport, e.g. in multi-compartment structures} - B01L 9/52: {Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips} - G01N 1/312: {for samples mounted on planar substrates} - G01N 35/10: Devices for transferring samples {or any liquids} to, in, or from, the analysis apparatus, e.g. suction devices, injection devices {(G01N35/0099 takes precedence)} - G02B 21/34: Microscope slides, e.g. mounting specimens on microscope slides - B01L 2300/06: Auxiliary integrated devices, integrated components - B01L 2300/0609: Holders integrated in container to position an object - B01L 2300/0848: Specific forms of parts of containers - B01L 2300/0861: Configuration of multiple channels and/or chambers in a single devices - G01N 1/2813: {Producing thin layers of samples on a substrate, e.g. smearing, spinning-on (G01N1/30 takes precedence)} - G01N 33/552: Glass or silica - G01N 2035/00138: {Slides} - G01N 2035/00158: {Elements containing microarrays, i.e. "biochip"} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - G02B: OPTICAL ELEMENTS, SYSTEMS OR APPARATUS ## Abstract The invention relates to a device for bringing an immobilised reactant into contact with at least one fluid, said device comprising: a basin having a bottom surface from which an upper side of at least one elevated portion projects into the interior of the basin, and said at least one elevated portion having a channel running, preferably perpendicular to the bottom surface of the basin, from the bottom surface of the basin to the upper side of the portion, and leading into at least one outlet opening on the upper side of the elevated portion, wherein the channel is fed from at least one fluid reservoir via a supply element by means of a pump device, said reservoir containing the at least one fluid; a holding device designed to detachably hold at least one object carrier, preferably a plurality of object carriers, having at least one surface of adhesion to the immobilised reactant, in such a way that the adhesion surface is facing the interior of the basin, and the upper side of the elevated portion and the adhesion surface are positioned relative to one another in such a way that fluid exiting the outlet opening comes into contact with the immobilised reactant; and optionally an object carrier introduced into the holding device. The invention also relates to a method for bringing an immobilised reactant into contact with at least one fluid, comprising the following steps: immobilising the reactant on the adhesion surface of at least one object carrier suitable for introduction into the holding device of the device according to the invention; (provided that the preceding steps were carried out outside of the device according to the invention) introducing the object carrier into the holding device of the device according to the invention, preferably via a transport device in the device according to the invention; introducing the at least one fluid via the supply element in the direction of the outlet opening, until the at least one fluid comes into contact with the immobilised reactant. ## Claims ### Claim at position 1 1: A device comprising: (i) a trough having a base from which at least one elevation having an upper side projects into the interior of the trough, wherein the at least one elevation has a channel which runs from the base of the trough to the upper side of the elevation, and opens into at least one escape opening on the upper side of the elevation, wherein the channel is fed from at least one liquid reservoir containing at least one liquid via a feed element by a pumping device, (ii) a holding device configured to detachably hold at least one microscope slide having at least one adhesion surface containing an immobilized reaction partner, such that the adhesion surface is facing the interior of the trough, and that the upper side of the elevation and the adhesion surface are positioned relative to one another such that liquid escaping from the escape opening is in contact with the immobilized reagent, and (iii) optionally one or more microscope slides inserted into the holding device, wherein the device is suitable for contacting the immobilized reaction partner with the at least one liquid. ### Claim at position 2 (depends on 1) 2: The device as claimed in claim 1, wherein the channel is additionally fed from a further liquid reservoir containing a second liquid. ### Claim at position 3 (depends on 1) 3: The device as claimed in claim 1, wherein the channel opens into more than one escape opening on the upper side of the elevation. ### Claim at position 4 (depends on 1) 4: The device as claimed in claim 1, wherein the adhesion surface has a central point and the upper side of the elevation has a central point, and wherein the central point of the adhesion surface and the central point of the upper side of the elevation are opposite each other. ### Claim at position 5 (depends on 1) 5: The device as claimed in claim 1, wherein the trough has at least one drain opening. ### Claim at position 6 (depends on 1) 6: The device as claimed in claim 1, wherein the holding device allows a movement of the microscope slide. ### Claim at position 7 (depends on 6) 7: The device as claimed in claim 6, wherein the trough is further provided with at least one air nozzle configured for drying the adhesion surface. ### Claim at position 8 (depends on 1) 8: The device as claimed in claim 1, wherein the pumping device allows any liquid which is in contact with the immobilized reaction partner to be aspirated from the direction of the escape opening. ### Claim at position 9 (depends on 1) 9: The device as claimed in claim 1, further comprising a transport device which allows the insertion of the microscope slide into the device. ### Claim at position 10 (depends on 1) 10: The device as claimed in claim 1, wherein the escape opening on the upper side of the elevation, the upper side of the elevation and the adhesion surface are designed such that the at least one liquid flows tangentially along the immobilized reaction partner when said liquid is introduced into the device. ### Claim at position 11 (depends on 1) 11: The device as claimed in claim 1, wherein the holding device comprises a frame equipped to accommodate and to hold at least one microscope slide, wherein, after accommodation, the adhesion surface is situated on that side of the microscope slide which is resting on the frame. ### Claim at position 12 12: A holding device comprising a frame equipped to accommodate at least one microscope slide, wherein the microscope slide has at least one adhesion surface containing an immobilized reaction partner, wherein, after accommodation, the adhesion surface of the microscope slide is situated on that side of the microscope slide which is resting on the frame, and wherein, after accommodation, the adhesion surface is positioned such that it is accessible to a contact with liquid escaping from the escape opening. ### Claim at position 13 (depends on 12) 13: The holding device as claimed in claim 12, further comprising the at least one microscope slide. ### Claim at position 14 (depends on 11) 14: The device as claimed in claim 11, wherein the frame has at least one lateral wall optionally provided with a handle. ### Claim at position 15 (depends on 14) 15: The device as claimed in claim 14, wherein the lateral wall of the frame has a profile suited to positioning the holding device and thus the adhesion surface in the device. ### Claim at position 16 (depends on 1) 16: A method for contacting an immobilized reaction partner with at least one liquid, comprising: introducing the at least one liquid to the device of claim 1, comprising at least one microscope slide containing the immobilized reaction partner, in the direction of the escape opening via the feed element until the at least one liquid is in contact with the immobilized reaction partner. ### Claim at position 17 (depends on 16) 17: The method as claimed in claim 16, further comprising: introducing a second liquid and optionally a further liquid in the direction of the escape opening via the feed element until the second liquid is in contact with the immobilized reaction partner. ### Claim at position 18 (depends on 16) 18: The method as claimed in claim 16, further comprising: introducing a wash solution in the direction of the escape opening via the feed element until the wash solution is in contact with the immobilized reaction partner, wherein the introducing of the wash solution can be carried out before and/or after the introducing of the at least one liquid. ### Claim at position 19 (depends on 18) 19: The method as claimed in claim 18, wherein the introducing of the wash solution is carried out until the device has been completely cleared of contaminants. ### Claim at position 20 (depends on 16) 20: The method as claimed in claim 16, wherein the introducing of the at least one liquid, the second liquid and optionally further liquid and/or wash solution is followed by aspirating the at least one or second and optionally further liquid or the wash solution from the immobilized reaction partner from the direction of the adhesion surface via the channel. ### Claim at position 21 (depends on 16) 21: The method as claimed in claim 16, wherein the at least one microscope slide is accommodated in the holding device, and the holding device is inserted into the device.