# US 2016/0257977 A1 · Enhanced Fermentation Process Using a Transglycosidase

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- Number: US 2016/0257977 A1
- Document ID: US20160257977A1
- Status: Published application
- Kind: A1
- Application number: 15/031,459
- Filed: 2014-10-21
- Published: 2016-09-08
- Earliest claimed: 2013-10-24
- Assignees: Danisco US Inc.
- Applicants: Danisco US Inc.
- Inventors: Gang Duan; Jayarama K. Shetty; Xin Wang; Hongxian Xu; Xiaoping Zhang; Peng Zhou
- Web page: https://patentgrep.com/patent/US20160257977A1

## CPC

- C12P 13/14: Glutamic acid; Glutamine
- C12P 7/48: Tricarboxylic acids, e.g. citric acid
- C12P 7/56: Lactic acid
- C12P 13/08: Lysine; Diaminopimelic acid; Threonine; Valine
- C12P 19/14: produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase}
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}

## Abstract

The present invention relates to methods for improving fermentation processes, including increasing product yield, reduced viscosity, and/or reduced foaming.

## Claims

### Claim 1

A method of making a fermentation product comprising;
fermenting a feedstock with a fermenting microorganism in a fermentation broth to make a fermentation product, wherein the fermenting comprises a transglycosidase, and wherein the fermentation product has a higher yield than a control fermentation lacking the transglycosidase.

### Claim 2 (depends on 1)

The method of claim 1 further comprising recovering the fermentation product.

### Claim 3 (depends on 1)

The method of claim 1 wherein the fermentation product is lysine, lactic acid, or glutamic acid.

### Claim 4 (depends on 1)

The method of claim 1 wherein the fermenting organism is Brevibacterium flavum, Corynebacterium glutamicum 10178, Corynebacterium glutamicum 10238, or Lactobacillus rhamnosus.

### Claim 5 (depends on 1)

The method of claim 1 wherein the transglycosidase comprises SEQ ID NO:1, or a polypeptide have at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or even at least 99% amino acid sequence identity to SEQ ID NO: 1.

### Claim 6 (depends on 1)

The method of claim 1 wherein the transglycosidase is produced in a Trichoderma.

### Claim 7 (depends on 1)

The method of claim 1 wherein the feedstock comprises starch liquifact, granular starch, pure glucose, pure sucrose, or sugar syrup.

### Claim 8 (depends on 1)

The method of claim 1 further comprising foam reduction and/or viscosity reduction in the fermentation broth.

### Claim 9 (depends on 1)

The method of claim 1 wherein the TrTG is present at 1-6 Kg/MT, 2-5 Kg/MT, or 2-4 Kg/MT.

### Claim at position 10

10-12. (canceled)

### Claim 13 (depends on 15)

A The method of claim 15, wherein
the fermenting comprises a TrTG Trichoderma transglucosidase, wherein the lysine has a higher yield compared to a control fermentation lacking the Trichoderma transglucosidase, and wherein the fermentation broth has reduced viscosity compared to a control fermentation lacking the Trichoderma transglucosidase.

### Claim 14 (depends on 15)

A The method of claim 15, wherein
the fermenting comprises an Aspergillus niger transglucosidase, wherein the lysine has a higher yield compared to a control fermentation lacking the Aspergillus niger transglucosidase.

### Claim 15

A method of making lysine comprising;
fermenting a feedstock with Brevibacterium flavum in a fermentation broth to make lysine, wherein the fermenting comprises a transglucosidase, wherein the lysine has a higher yield compared to a control fermentation lacking the transglucosidase; or
fermenting a feedstock with Brevibacterium flavum in a fermentation broth to make lysine, wherein the fermenting comprises a betaglucanase, xylanase and cellulase enzyme complex, wherein the lysine has a higher yield compared to a control fermentation lacking the betaglucanase, xylanase and cellulase enzyme complex.

### Claim 16 (depends on 14)

The method according to claim 14, wherein the feedstock comprises pure glucose or sugar syrup.

### Claim 17

(canceled)

### Claim 18 (depends on 14)

The method according to claim 14 further comprising foam reduction and/or viscosity reduction.

### Claim 19

A method of making glutamic acid comprising;
fermenting a feedstock with Corynebacterium glutamicum SP 10238 in a fermentation broth to make glutamic acid, wherein the fermenting comprises a TrTG, wherein the fermentation broth has reduced viscosity compared to a control fermentation lacking the TrTG, and wherein the fermentation broth has reduced foam compared to a control fermentation lacking the TrTG; or
fermenting a feedstock with Corynebacterium glutamicum SP 10238 in a fermentation broth to make glutamic acid, wherein the fermenting comprises an exoglucanase, a endoglucanase, a β-glucosidase, and a xylanase, wherein the fermentation broth has reduced viscosity compared to a control fermentation lacking the exoglucanase, the endoglucanase, the β-glucosidase, and the xylanase, and wherein the fermentation broth has reduced viscosity compared to a control fermentation lacking the exoglucanase, the endoglucanase, the β-glucosidase, and the xylanase.

### Claim 20

(canceled)

### Claim 21

A method of making lactic acid comprising;
fermenting a feedstock with Lactobacillus rhamnosus in a fermentation broth to make lactic acid, wherein the fermenting comprises a TrTG, wherein the lactic acid has a higher yield compared to a control fermentation lacking the TrTG, and wherein the fermentation broth has reduced viscosity compared to a control fermentation lacking the TrTG; or
fermenting a feedstock with Lactobacillus rhamnosus in a fermentation broth to make lactic acid, wherein the fermenting comprises a betaglucanase, xylanase and cellulase enzyme complex, wherein the lactic acid has a higher yield compared to a control fermentation lacking the betaglucanase, xylanase and cellulase enzyme complex, and wherein the fermentation broth has reduced viscosity compared to a control fermentation lacking the betaglucanase, xylanase and cellulase enzyme complex.

### Claim 22 (depends on 21)

The method according to claim 21, wherein the feedstock comprises pure glucose or sugar syrup.

### Claim at position 21

23-35. (canceled)

### Claim 36 (depends on 15)

The method according claim 15, wherein the feedstock comprises pure glucose or sugar syrup.

### Claim 37 (depends on 15)

The method according to claim 15, further comprising foam reduction and/or viscosity reduction.

### Claim 38 (depends on 17)

The method according to claim 17, further comprising foam reduction and/or viscosity reduction.