# US 2016/0257941 A1 · Sulfated Cellulose Hydrate Membrane, Method for Producing Same, and Use of the Membrane as an Adsorption Membrane for a Virus Purification Process

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- Number: US 2016/0257941 A1
- Document ID: US20160257941A1
- Status: Published application
- Kind: A1
- Application number: 15/029,203
- Filed: 2014-09-11
- Published: 2016-09-08
- Earliest claimed: 2013-10-14
- Assignees: Sartorius Stedim Biotech GmbH
- Applicants: Sartorius Stedim Biotech GmbH
- Inventors: Louis Villain; Hans-Heinrich Hörl; Christian Brumm
- Web page: https://patentgrep.com/patent/US20160257941A1

## CPC

- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- B01D 67/00931: {by introduction of specific groups after membrane formation, e.g. by grafting}
- B01D 69/02: characterised by their properties
- B01D 71/20: Esters of inorganic acids, e.g. cellulose nitrate
- B01D 2323/30: Cross-linking
- B01D 2325/12: Adsorbents being present on the surface of the membranes or in the pores
- B01D 2325/24: Mechanical properties, e.g. strength
- C12N 2760/16051: Methods of production or purification of viral material
- C12N 2760/16151: Methods of production or purification of viral material
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- B01D: SEPARATION (separating solids from solids by wet methods B03B, B03D; by pneumatic jigs or tables B03B; by other dry methods B07; magnetic or electrostatic separation of solid materials from solid materials or fluids, separation by high-voltage electric fields B03C; centrifuges, vortex apparatus B04; presses per se for squeezing-out liquid from liquid-containing material B30B9/02; treatment of water C02F, e.g. softening by ion-exchange C02F1/42; {arrangements of air intake cleaners in gas turbine plants F02C7/05} ; arrangements or mounting of filters in air-conditioning, air-humidification or ventilation F24F13/28)

## Abstract

The present invention relates to a sulfated cellulose hydrate membrane, a method for the preparation thereof and the use of the membrane as adsorption membrane for the purification of viruses.

## Claims

### Claim 1

A sulfated cellulose hydrate membrane comprising a crosslinked cellulose hydrate matrix with pores which extend from one main surface to another main surface of the membrane, wherein the cellulose hydrate membrane has sulfate ligands on its inner and outer surfaces for adsorptive substance separation.

### Claim 2 (depends on 1)

The sulfated cellulose membrane as claimed in claim 1, wherein the degree of sulfation of the cellulose hydrate matrix is 10% by weight or more.

### Claim 3 (depends on 1)

The sulfated cellulose hydrate membrane a claimed in claim 1, wherein the mean pore size of the membrane is between 0.5 and 5.0 μm.

### Claim 4 (depends on 13)

The sulfated cellulose hydrate membrane as claimed in claim 13, wherein the degree of crosslinking of the cellulose hydrate matrix is 0.05 to 0.5.

### Claim 5 (depends on 1)

A method for preparing a sulfated cellulose hydrate membrane as claimed in claim 1, comprising the steps of:
providing a cellulose membrane with a pore size of 0.1 to 20 μm;
crosslinking the cellulose hydrate matrix using a crosslinker having at least two functional groups in the molecule which react with the hydroxyl groups of the cellulose hydrate matrix; and
sulfating the crosslinked cellulose hydrate matrix.

### Claim 6 (depends on 5)

The method as claimed in claim 5, wherein the crosslinker is selected from the group consisting of diepoxide compounds, diisocyanates, epichlorohydrin, epibromohydrin, dimethylurea, dimethylethyleneurea, dimethylchlorsilan, bis(2-hydroxyethylsulfone), divinylsulfone, alkylene dihalides, hydroxyalkylene dihalides and diglycidyl ethers or a mixture thereof.

### Claim 7 (depends on 5)

The method as claimed in claim 5, wherein 1,4-butanediol diglycidyl ether or ethylene glycol diglycidyl ether is used as crosslinker.

### Claim 8 (depends on 5)

The method as claimed in claim 5, wherein the concentration of the crosslinker in the crosslinking solution of 10 to 30% by weights.

### Claim 9 (depends on 5)

The method as claimed in claim 5, wherein the sulfation is effected by reacting the crosslinked cellulose hydrate matrix having a Lewis base-SO3 complex.

### Claim 10 (depends on 5)

The method as claimed in claim 5, wherein the sulfation is effected by reaction with a SO3-pyridine complex.

### Claim 11 (depends on 10)

The method as claimed in claim 10, wherein the concentration of SO3-pyridine-complex in the sulfation solution is 1 to 40% by weight.

### Claim 12 (depends on 5)

The method as claimed in claim 5, wherein the sulfation is effected at a temperature of 20 to 90° C.

### Claim 13 (depends on 1)

A method of purifying viruses or virus fragments comprising:
preparing a sulfated cellulose hydrate membrane as claimed in claim 1; and
contacting the membrane with a solution containing the viruses or virus fragments.

### Claim 14 (depends on 13)

The method of claim 13 wherein the viruses have a molecular mass of greater than 107 Da.

### Claim 15 (depends on 13)

The method of claim 13 wherein the viruses are influenza viruses.