# US 2016/0257935 A1 · Method for Differentiating Pluripotent Stem Cell Induced from Mesenchymal Stem Cell into Osteoblast

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 2016/0257935 A1
- Document ID: US20160257935A1
- Status: Published application
- Kind: A1
- Application number: 15/033,393
- Filed: 2013-11-05
- Published: 2016-09-08
- Earliest claimed: 2013-11-01
- Assignees: Bbhc Co., Ltd.
- Applicants: Bbhc Co., Ltd.
- Inventors: Sang Yeon LEE; Won Ju JUNG; Ho Bin KIM; Min Sun OH; Kye Ho LEE
- Web page: https://patentgrep.com/patent/US20160257935A1

## CPC

- C12N 5/0654: {Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth}
- A61K 35/32: Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
- A61P 19/00: Drugs for skeletal disorders
- C12N 5/0696: {Artificially induced pluripotent stem cells, e.g. iPS}
- C12N 2500/38: Vitamins
- C12N 2500/42: Organic phosphate, e.g. beta glycerophosphate
- C12N 2500/76: from plants
- C12N 2501/155: Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
- C12N 2501/39: Steroid hormones
- C12N 2501/999: Small molecules not provided for elsewhere
- C12N 2506/1369: from blood-borne mesenchymal stem cells, e.g. MSC from umbilical blood
- C12N 2506/45: from artificially induced pluripotent stem cells
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The present invention relates to a medium composition containing an Ecklonia cava extract for dedifferentiation an induced pluripotent stem cell. Also, the present invention relates to a method for differentiating an induced pluripotent stem cell produced by using the medium composition into osteoblasts. When using the medium composition according to the present invention, induced pluripotent stem cells using mesenchymal stem cells can be produced efficiently, and the pluripotent stem cells which have been produced can be useful as a cell treatment agent by being capable of being differentiated into osteoblasts.

## Claims

### Claim 1

A method for differentiating mesenchymal stem cells into osteoblasts, comprising the steps of:
(a) adding an Ecklonia cava extract to a cell culture medium;
(b) dedifferentiating mesenchymal stem cells into induced pluripotent stem cells in the medium; and
(c) differentiating the induced pluripotent stem cells into osteoblasts.

### Claim 2 (depends on 1)

The method of claim 1, wherein the Ecklonia cava extract is added to a medium selected from the group consisting of DMEM (Dulbecco's Modified Eagle's Medium), MEM (Minimal Essential Medium), BME (Basal Medium Eagle), RPMI 1640, F-10, F-12, DMEM-F12, α-MEM (α-Minimal Essential Medium), G-MEM (Glasgow's Minimal Essential Medium), IMDM (Iscove's Modified Dulbecco's Medium), MacCoy's 5A medium, AmnioMax complete Medium, AminoMax II complete Medium, Chang's Medium and MesemCult-XF Medium.

### Claim 3 (depends on 1)

The method of claim 1, wherein the Ecklonia cava extract is added to the medium in an amount of 100-400 μg/ml.

### Claim 4 (depends on 1)

The method of claim 1, wherein the medium further contains 0.01-10 v/v % of energy water.

### Claim 5 (depends on 1)

The method of claim 1, wherein step c) is performed using a medium containing dexamethason, β-glycerol phosphate, ascorbic acid and bone morphogenic protein (BMP).

### Claim 6 (depends on 5)

The method of claim 5, wherein the medium contains 3-isobutyl-1-methylxanthine, hydrocortisone and indomethacin in a medium selected from the group consisting of DMEM (Dulbecco's Modified Eagle's Medium), MEM (Minimal Essential Medium), BME (Basal Medium Eagle), RPMI 1640, F-10, F-12, DMEM-F12, α-MEM (α-Minimal Essential Medium), G-MEM (Glasgow's Minimal Essential Medium), IMDM (Iscove's Modified Dulbecco's Medium), MacCoy's 5A medium, AmnioMax complete Medium, AminoMax II complete Medium, Chang's Medium and MesemCult-XF Medium.

### Claim 7 (depends on 1)

Osteoblasts produced by the method of claim 1.

### Claim 8 (depends on 7)

A cell therapy composition comprising the osteoblasts of claim 7.

### Claim 9

A medium composition comprising an Ecklonia cava extract for dedifferentiating mesenchymal stem cells into induced pluripotency stem cells, characterized by the induced pluripotency stem cells are capable of differentiating into osteoblasts.