# US 2016/0252537 A1 · System and method for processing of biological tissue samples > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2016/0252537 A1 - Document ID: US20160252537A1 - Status: Published application - Kind: A1 - Application number: 15/032,845 - Filed: 2014-11-12 - Published: 2016-09-01 - Earliest claimed: 2013-11-13 - Assignees: Stempeutics Research Private Limited - Applicants: Stempeutics Research Private Limited - Inventors: Cherat MURALI; Damle ALOK; Sundar Raj SWATHI; Majumdar ANISH SEN; Deshmukh ABHIJEET; Sathya Kumar BYALAPPA MANJUNATH; Lohidhakshan PRAJOD THIRUVAMPATTIL - Web page: https://patentgrep.com/patent/US20160252537A1 ## CPC - C12M 23/50: {Means for positioning or orientating the apparatus (C12M41/08 takes precedence)} - G01N 35/0099: {comprising robots or similar manipulators (robots per se B25J)} - C12M 41/48: {Automatic or computerized control (automatic analysis G01N35/00)} - C12M 45/02: {by mechanical forces; Stirring; Trituration; Comminuting (crushing, pulverizing, disintegrating in general B02C)} - C12M 45/09: {by enzymatic treatment} - C12M 47/04: {Cell isolation or sorting (purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04)} - C12N 5/069: {Vascular Endothelial cells} - G01N 35/02: using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations {(G01N35/0098 and G01N35/0099 take precedence)} - G01N 2035/00346: {Heating or cooling arrangements} - G01N 2035/00891: {Displaying information to the operator} - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract The present disclosure provides an automated system for processing of tissue. The system comprises a plurality of containers for storing at least one of tissue samples, buffer solutions, enzymes and other reagents, tissue processing container for processing of the tissue, and a robotic assembly coupled to the tissue processing container. The robotic assembly is configured to: carry the tissue processing container towards each of the plurality of containers, and align an inlet port of the tissue processing container with an outlet port of each of the plurality of containers for collecting the liquids, and moves the tissue processing container in multiple-planes to perform at least one of the washing processes, digestion process, phase separation process and combination thereof. The system also comprises of a control unit interfaced with the robotic assembly for controlling operations of the robotic assembly while processing the tissue. ## Claims ### Claim 1 An automated system (500) for processing of tissue, said system (500) comprising: a plurality of containers (505), wherein each of the plurality of containers (505) stores at least one of tissue samples, buffer solutions, enzymes and reagents; a tissue processing container (703) for processing of the tissue; a robotic assembly (100) coupled to the tissue processing container (703), wherein the robotic assembly (100) is configured to: carry the tissue processing container (703) towards each of the plurality of containers (505), and align an inlet port of the tissue processing container (703) with an outlet port of each of the plurality of containers (505) for collecting at least one of tissue samples, buffer solutions, enzymes and reagents; and moves the tissue processing container (703) in multiple-planes to perform at least one of the washing processes, digestion process, phase separation process and combination thereof for separating an aqueous fraction and a fatty fraction from the digested tissue samples; and a control unit (511) interfaced with the robotic assembly (100) for controlling operations of the robotic assembly (100) while processing the tissue. ### Claim 2 (depends on 1) The system (500) as claimed in claim 1, wherein the tissues are mammalian tissues selected from at least one of adipose tissue, placental tissue and umbilical cord tissue. ### Claim 3 (depends on 2) The system (500) as claimed in claim 2 isolates Stromal Vascular Fraction (SVF) cells by processing the adipose tissue, and multi-potent stem/stromal cells from placental and umbilical cord tissue. ### Claim 4 (depends on 1) The system (500) as claimed in claim 1 further comprises a cell concentration unit (507) configured, to filter the aqueous fraction of digested tissue for isolating cells, wherein the cell concentration unit (507) receives the aqueous fraction tissue sample from the tissue processing container (703). ### Claim 5 (depends on 4) The system (500) as claimed in claim 4, wherein the cell concentration unit (507) is at least one of filter assembly, spinner and a centrifugation assembly. ### Claim 6 (depends on 1) The system (500) as claimed in claim 1 comprises a waste collection unit (508) for receiving at least one of aqueous fraction of tissues and fatty fraction of tissues from the tissue processing container (703). ### Claim 7 (depends on 1) The system (500) as claimed in claim 1 is enclosed in a chamber (503). ### Claim 8 (depends on 1) The system (500) as claimed in claim 1, wherein the control unit (511) is provided with a user interface having a display unit (506) and input buttons to feed in required parameters for processing the tissue. ### Claim 9 (depends on 1) The system as claimed in claim 1 comprises at least one temperature sensor (504), placed in a chamber (503) to measure and regulate the temperature of the chamber (503), wherein the temperature sensor (504) is interfaced with the control unit (511). ### Claim 10 (depends on 1) The system (500) as claimed in claim 1 comprises at least one heating element (502) placed in a chamber, wherein the heating element (502) is interfaced with the control unit (511) to maintain the temperature of the chamber (503) within a predetermined limit, and heat the tissue processing container (703). ### Claim 11 (depends on 1) The system (500) as claimed in claim 1, wherein the outlet port of each of the plurality of containers and the inlet port of the tissue processing container are provided with a valve assembly (515). ### Claim 12 (depends on 11) The system (500) as claimed in claim 11 wherein the valve assemblies (515) are interfaced with the control unit (511). ### Claim 13 (depends on 1) The system (500) as claimed in claim 1 comprises a wash container (514) adapted to be coupled to the robotic assembly (100). ### Claim 14 (depends on 13) The system (500) as claimed in claim 13, wherein the robotic assembly (100) is configured to: carry the wash container (514) towards the plurality of containers (505), and align an inlet port of the wash container (514) with an outlet port of a container which is storing the wash buffer solution for collecting the wash buffer solution; and carry the wash container (514) towards a cell concentration unit (507), and align the outlet port of the wash container (514) with an inlet port of the cell concentration unit (507) for supplying the wash buffer solution to the cell concentration unit (507) for washing the aqueous fraction tissue sample. ### Claim 15 (depends on 1) The system (500) as claimed in claim 1, wherein each of the plurality of containers (505) and the tissue processing container (703) are equipped with a radio frequency identification tags. ### Claim 16 (depends on 1) The system (500) as claimed in claim 1, wherein the robotic assembly (100) comprises plurality of sensors (S). ### Claim 17 (depends on 16) The system as claimed in claim 16, wherein the plurality of sensors (S) are interfaced with the control unit (511) to control the movement of the robotic assembly (100) in a predefined path. ### Claim 18 (depends on 1) A method for processing of tissue using the automated system (500) as claimed in claim 1, said method comprises acts of: a. receiving tissue samples and wash buffer solution to a tissue processing container (703), wherein the robotic assembly aligns inlet ports of the tissue processing container (703) to outlet ports of the containers storing tissues and wash buffer solution; b. washing the tissue samples with wash buffer solution by agitating the mixture in the tissue processing container with the help of robotic assembly, wherein the robotic assembly moves the tissue processing container in multiple planes; c. allowing phase separation of the mixture to obtain an initial fatty upper fraction and an initial aqueous lower fraction in the tissue processing container, wherein the phase separation is carried out by tilting the tissue processing container by 90 degrees with respect to X axis by a robotic assembly; d. disposing the initial lower aqueous fraction obtained in step (c) to a waste collection unit, wherein the robotic assembly aligns inlet ports of waste collection unit with an outlet port of the tissue processing container; e. receiving a predetermined quantity of a digestive buffer contained in a digestive buffer container to the tissue processing container, wherein the robotic assembly aligns inlet ports of the tissue processing container (703) to outlet ports of the container storing digestive buffer solution; f. digesting the fatty upper fraction with the digestive buffer by agitating the mixture in the tissue processing container with the help of robotic assembly, wherein the robotic assembly moves the tissue processing container in multiple planes; g. allowing phase separation of the mixture in the tissue processing container to obtain a final fatty upper fraction and a final aqueous lower fraction, wherein the phase separation is carried out by tilting the tissue processing container by 90 degrees with respect to X axis by a robotic assembly. ### Claim 19 (depends on 18) The method as claimed in claim 18 comprises act of detecting position of the robotic assembly (100) by sensors, and regulating the robotic unit (100) by the control unit (511) to carry the tissue processing container toward each of the plurality of containers and the water collection unit. ### Claim 20 (depends on 18) The method as claimed in claim 18 comprises act of detecting the alignment of tissue processing container with at least one of, the plurality of containers and waste collection unit by a control unit, and operating the valve assembly. ### Claim 21 (depends on 1) An automated system (500) as claimed in claim 1 is used for processing of biological tissue samples.