# US 2016/0252484 A1 · System and method for modified gas chromatographic data analysis > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2016/0252484 A1 - Document ID: US20160252484A1 - Status: Published application - Kind: A1 - Application number: 15/027,897 - Filed: 2014-10-08 - Published: 2016-09-01 - Earliest claimed: 2013-10-09 - Assignees: Spectrosense Ltd. - Applicants: Spectrosense Ltd. - Inventors: Avi RUBINSTEIN - Web page: https://patentgrep.com/patent/US20160252484A1 ## CPC - G01N 30/8637: {Peak shape} - G01N 30/8689: {Peak purity of co-eluting compounds} - G01N 30/8693: {Models, e.g. prediction of retention times, method development and validation} - G16H 50/50: for simulation or modelling of medical disorders - G16Z 99/00: Subject matter not provided for in other main groups of this subclass - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - G16H: HEALTHCARE INFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR THE HANDLING OR PROCESSING OF MEDICAL OR HEALTHCARE DATA - G16Z: INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS, NOT OTHERWISE PROVIDED FOR ## Abstract A method that employs self-reliant gas chromatography for determining a measure of match between acquired gas chromatographic data representative of a sample and reference gas chromatographic data, the acquired gas chromatographic data includes at least one observed chromatographic peak, the reference gas chromatographic data includes at least one reference chromatographic peak, the at least one observed chromatographic peak and the at least one reference chromatographic peak are characterized by at least one temporal attribute and at least one shape attribute, the method includes at least the procedure of: estimating respectively, for said at least one observed chromatographic peak, said measure of match according to a degree of fitness between an observed value and respective a reference value of said at least one shape attribute. ## Claims ### Claim 1 A method that employs self-reliant gas chromatography for determining a measure of match between acquired gas chromatographic data representative of a sample and reference gas chromatographic data, the acquired gas chromatographic data includes at least one observed chromatographic peak, the reference gas chromatographic data includes at least one reference chromatographic peak, the at least one observed chromatographic peak and the at least one reference chromatographic peak are characterized by at least one temporal attribute and at least one shape attribute, the method comprising the procedures of: determining respectively, for said at least one observed chromatographic peak, at least one parameter in a modeling function, such to substantially fit said modeling function to said at least one observed chromatographic peak, said at least one parameter includes at least one of said at least one shape attribute; associating respectively, for said at least one observed chromatographic peak said at least one reference chromatographic peak according to: a degree of correspondence between an observed value of said at least one shape attribute of said at least one observed chromatographic peak, and a reference value of respective said at least one shape attribute of said at least one reference chromatographic peak; and a degree of correspondence between an observed value of said at least one temporal attribute of said at least one observed chromatographic peak, and a reference value of respective said at least one reference temporal attribute of said at least one reference chromatographic peak; and estimating respectively, for said at least one observed chromatographic peak, said measure of match according to a degree of fitness between said observed value and respective said reference value of said at least one shape attribute, according to said procedure of associating. ### Claim 2 (depends on 1) The method according to claim 1, wherein said procedure of estimating is further according to a degree of fitness between said observed value and said reference value of corresponding said at least one temporal attribute. ### Claim 3 (depends on 1) The method according to claim 1, further comprising a procedure of representing, for said at least one observed chromatographic peak, in a coordinate system whose first coordinate is said at least one shape attribute and whose second coordinate is said at least one temporal attribute, a respective observed data item having a first coordinate that is said observed value of said at least one shape attribute, and a second coordinate that is said observed value of said at least one temporal attribute, such to define a position of said observed data item in said coordinate system. ### Claim 4 (depends on 3) The method according to claim 3, further comprising a procedure of representing in said coordinate system, for said at least one reference chromatographic peak, a respective reference data item having a first coordinate that is said reference value of said at least one shape attribute and a second coordinate that is said reference value of said at least one temporal attribute, such to define a position of said reference data item is said coordinate system. ### Claim 5 (depends on 4) The method according to claim 4, further comprising a procedure of identifying, in said coordinate system, at least one reference data item cluster that includes a plurality of reference data items all of whose respective reference chromatographic peaks are associated with a biomarker that is indicative of either one of a healthy medical condition, and adverse medical condition, and an indeterminate medical condition of a subject from whom said sample is acquired. ### Claim 6 (depends on 5) The method according to claim 5, further comprising a procedure of determining for said at least one observed data item in said coordinate system, whether its respective said observed chromatographic peak is associated with at least one said biomarker that is indicative of either one of a healthy medical condition, an adverse medical condition, and an indeterminate medical condition, according to said position of said at least one observed data item in said coordinate system in relation to a defined boundary of said at least one reference data item cluster in said coordinate system. ### Claim 7 (depends on 1) The method according to claim 1, further comprising a procedure of constructing a database of said reference gas chromatographic data, acquired from a plurality of compounds, where each compound is acquired from a source that is known to be associated with either one of a healthy medical condition, and an adverse medical condition. ### Claim 8 (depends on 6) The method according to claim 6, wherein said procedure of determining is based on the number of accumulated occurrences in each of said position of respective said observed data item in relation to said defined boundary of said reference data item cluster. ### Claim 9 (depends on 7) The method according to claim 7, further comprising a procedure of establishing a set of criteria to define which said reference data item constitutes at least part of said reference data item cluster. ### Claim 10 (depends on 1) The method according to claim 1, wherein said at least one shape attribute is selected from a list consisting of: a characteristic shape parameter in said modeling function; a characteristic scale parameter in said modeling function; a maximum value of at least one of said probability distribution functions; a mean parameter of said modeling function; a rate parameter in said modeling function; a variance of said modeling function; a degree of asymmetry of said probability distribution function; slopes of said probability distribution function at certain points in time; and at least one constant in said modeling function. ### Claim 11 (depends on 2) The method according to claim 2, further comprising a procedure of defining an N-dimensional data space, where at least one dimension corresponds with said at least one temporal attribute, and each of the remaining dimensions in said N-dimensional data space respectively correspond with said at least one shape attribute. ### Claim 12 (depends on 11) The method according to claim 11, further comprising a procedure of representing said at least one observed chromatographic peak as respective observed data item and said at least one reference chromatographic peak as respective reference data item in said N-dimensional data space. ### Claim 13 (depends on 12) The method according to claim 12, further comprising a procedure of performing statistical analysis on said acquired gas chromatographic data in said N-dimensional data space so as to assess whether said observed chromatographic peak is associated with at least one biomarker that is indicative of either one of a healthy medical condition, an adverse medical condition, and an indeterminate medical condition, associated with a subject from whom said sample is acquired. ### Claim 14 (depends on 1) The method according to claim 1, wherein a threshold value is defined for said degree of correspondence between said observed value and said reference value of said at least one temporal attribute, where if said degree of correspondence is above said threshold value it is supposed that there is no association between respective said observed chromatographic peak and said reference chromatographic peak, and if said degree of correspondence is below said threshold value it is supposed that there is an association between respective said reference chromatographic peak and said observed chromatographic peak. ### Claim 15 (depends on 13) The method according to claim 13, wherein said statistical analysis is facilitated by at least one decision rule that is based on the incidence of correspondences, between said observed data item and said reference data item, according to at least one statistical criterion. ### Claim 16 A self-reliant gas chromatography system for analysis of gas chromatographic data, the system comprising: a chromatographic separation column for separating a sample into a plurality of constituents, said chromatographic separation column includes an inlet and an outlet; a sample delivery device coupled with said chromatographic separation column at said inlet, for providing said sample to said chromatographic separation column; a detector in communication with said outlet of said chromatographic separation column for detecting at least a portion of said plurality of constituents, said detector producing a signal that includes said gas chromatographic data corresponding to characteristics of the detected said at least a portion of said sample, said gas chromatographic data including at least one observed chromatographic peak characterized by at least one temporal attribute and at least one observed shape attribute; a memory device for storing said gas chromatographic data and a plurality of gas chromatographic reference data, said gas chromatographic reference data including at least one reference chromatographic peak characterized by at least one temporal attribute and at least one reference shape attribute; and a processor coupled with said detector and with said memory device, said processor determines respectively, for said at least one observed chromatographic peak, at least one parameter in a modeling function, such to substantially fit said modeling function to said at least one observed chromatographic peak, said at least one parameter includes at least one of said at least one shape attribute, said processor associates respectively, for said at least one observed chromatographic peak said at least one reference chromatographic peak according to: a degree of correspondence between an observed value of said at least one shape attribute of said at least one observed chromatographic peak, and a reference value of respective said at least one shape attribute of said at least one reference chromatographic peak; and a degree of correspondence between an observed value of said at least one temporal attribute of said at least one observed chromatographic peak, and a reference value of respective said at least one reference temporal attribute of said at least one reference chromatographic peak; and said processor estimates respectively, for said at least one observed chromatographic peak, said measure of match according to a degree of fitness between said observed value and respective said reference value of said at least one shape attribute. ### Claim 17 (depends on 16) The system according to claim 16, wherein said processor said estimates further according to a degree of fitness between said observed value and said reference value of corresponding said at least one temporal attribute. ### Claim 18 (depends on 16) The system according to claim 16, wherein said processor further represents, for said at least one observed chromatographic peak, in a coordinate system whose first coordinate is said at least one shape attribute and whose second coordinate is said at least one temporal attribute, a respective observed data item having a first coordinate that is said observed value of said at least one shape attribute, and a second coordinate that is said observed value of said at least one temporal attribute, such to define a position of said observed data item in said coordinate system. ### Claim 19 (depends on 18) The system according to claim 18, wherein said processor further represents in said coordinate system, for said at least one reference chromatographic peak, a respective reference data item having a first coordinate that is said reference value of said at least one shape attribute and a second coordinate that is said reference value of said at least one temporal attribute, such to define a position of said reference data item is said coordinate system. ### Claim 20 (depends on 19) The system according to claim 19, wherein said processor further identifies, in said coordinate system, at least one reference data item cluster that includes a plurality of reference data items all of whose respective reference chromatographic peaks are associated with a biomarker that is indicative of either one of a healthy medical condition, and adverse medical condition, and an indeterminate medical condition of a subject from whom said sample is acquired. ### Claim 21 (depends on 20) The system according to claim 20, wherein said processor determines for said at least one observed data item in said coordinate system, whether its respective said observed chromatographic peak is associated with at least one said biomarker that is indicative of either one of a healthy medical condition, an adverse medical condition, and an indeterminate medical condition, according to said position of said at least one observed data item in said coordinate system in relation to a defined boundary of said at least one reference data item cluster in said coordinate system. ### Claim 22 (depends on 16) The system according to claim 16, wherein said processor constructs a database in said memory device said reference gas chromatographic data, acquired from a plurality of compounds, where each compound is acquired from a source that is known to be associated with either one of a healthy medical condition, and an adverse medical condition. ### Claim 23 (depends on 21) The system according to claim 21, wherein said processor determines for said at least one observed data item in said coordinate system, whether its respective said observed chromatographic peak is associated with at least one said biomarker that is indicative of either one of a healthy medical condition, an adverse medical condition, and an indeterminate medical condition according to the number of accumulated occurrences in each of said position of respective said observed data item in relation to said defined boundary of said reference data item cluster. ### Claim 24 (depends on 16) The system according to claim 16, wherein said processor establishes a set of criteria to define which said reference data item constitutes at least part of said reference data item cluster. ### Claim 25 (depends on 16) The system according to claim 16, wherein said at least one shape attribute is selected from a list consisting of: a characteristic shape parameter in said modeling function; a characteristic scale parameter in said modeling function; a maximum value of at least one of said probability distribution functions; a rate parameter in said modeling function; a variance of said modeling function; a degree of asymmetry of said probability distribution function; slopes of said probability distribution function at certain points in time; and at least one constant in said modeling function. ### Claim 26 (depends on 17) The system according to claim 17, wherein an N-dimensional data space is defined, where at least one dimension corresponds with said at least one temporal attribute, and each of the remaining dimensions in said N-dimensional data space respectively correspond with said at least one shape attribute. ### Claim 27 (depends on 26) The system according to claim 26, wherein said processor represents at least one observed chromatographic peak as respective observed data item, and said at least one reference chromatographic peak as respective reference data item in said N-dimensional data space. ### Claim 28 (depends on 27) The system according to claim 27, wherein said processor performs statistical analysis on said acquired gas chromatographic data in said N-dimensional data space so as to assess whether said observed chromatographic peak is associated with at least one biomarker that is indicative of either one of a healthy medical condition, an adverse medical condition, and an indeterminate medical condition, associated with a subject from whom said sample is acquired. ### Claim 29 (depends on 16) The system according to claim 16, wherein a threshold value is defined for said degree of correspondence between said observed value and said reference value of said at least one temporal attribute, where if said degree of correspondence is above said threshold value it is supposed that there is no association between respective said observed chromatographic peak and said reference chromatographic peak, and if said degree of correspondence is below said threshold value it is supposed that there is an association between respective said reference chromatographic peak and said observed chromatographic peak. ### Claim 30 (depends on 28) The system according to claim 28, wherein said statistical analysis is facilitated by at least one decision rule that is based on the incidence of correspondences, between said observed data item and said reference data item, according to at least one statistical criterion.