# US 2016/0251684 A1 · Utilization of intestinal bacteria from slaughtered animals in industrial processes and waste treatment > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2016/0251684 A1 - Document ID: US20160251684A1 - Status: Published application - Kind: A1 - Application number: 15/027,989 - Filed: 2014-10-08 - Published: 2016-09-01 - Earliest claimed: 2013-10-08 - Inventors: Eino Elias Hakalehto - Web page: https://patentgrep.com/patent/US20160251684A1 ## CPC - C12P 7/52: Propionic acid; Butyric acids - C12M 29/00: {Means for introduction, extraction or recirculation of materials, e.g. pumps (pumps per se F04B)} - C12P 7/04: acyclic - C12P 7/18: polyhydric - C12P 7/40: containing a carboxyl group {including Peroxycarboxylic acids} - C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)} - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} ## Abstract Slaughter waste normally contains considerable amounts of so-called stomach manure. Its biotechnical treatment rapidly decreases the waste problem. With the help of the method and device according to this invention stomach manure is first used as a biocatalyst in a process that produces mannitol. After that the lactic acid that at the same time farms into the slaughter waste is converted with the help of microbes into propionic acid or other useful compounds. ## Claims ### Claim at position 1 1-17 (canceled) ### Claim 18 A method of utilizing intestinal bacteria, the method comprising the steps of: preparing a process liquid by adding a sugar selected from the group consisting of monosaccharides, disaccharides, and their polymers into manure selected from the group consisting of stomach manure of a slaughtered animal, manure produced by an animal, and refinery sludge; and increasing productivity or production levels of desired biotechnical products in the process liquid. ### Claim 19 (depends on 18) The method according to claim 18, wherein the sugar is sucrose, glucose, fructose, and a mixture of any of sucrose, glucose and fructose. ### Claim 20 (depends on 19) The method according to claim 19, wherein the sugar is glucose and fructose that is produced enzymatically as a result of decomposition of starch, cellulose, hemicellulose or other macromolecule. ### Claim 21 (depends on 20) The method according to claim 20, wherein that an amount of the fructose is added by enzymatic means. ### Claim 22 (depends on 21) The method according to claim 21 further comprises the step of converting the sugar in the manure into mannitol using lactic acid bacteria in the manure. ### Claim 23 (depends on 22) The method according to claim 22 further comprises the step of lowering a pH value of the process liquid by changing the glucose into lactic acid by at least the lactic acid bacteria and microbes performing acetic acid fermentation, which simultaneously prevents decomposition or metabolization of the mannitol that has formed into the process liquid. ### Claim 24 (depends on 23) The method according to claim 23, wherein the pH value starts to be raised by adding a base when at least most of the fructose has converted into mannitol. ### Claim 25 (depends on 24) The method according to claim 24 further comprises the step of removing the mannitol by crystallization prior to raising the pH value. ### Claim 26 (depends on 25) The method according to claim 25 further comprises the step of heating the process liquid by leading a gas emission containing one of CO and CO2 into the process liquid, wherein the gas emission is formed from a combustion reaction. ### Claim 27 (depends on 26) The method according claim 26 further comprises the step of converting the lactic acid formed by the acetic acid fermentation to propionate. ### Claim 28 (depends on 27) The method according to claim 27, wherein the propionate is propionic acid, and wherein Clostridium bacteria is used in converting the propionic acid into an organic acid or an alcohol. ### Claim 29 (depends on 27) The method according to claim 27 further comprises the step of adding at least one bacterial strain into the manure for assisting in changing a part of the lactic acid into the propionate, wherein the part of the lactic acid is collected for burning or for chemical utilization as a preservative in secondary products. ### Claim 30 (depends on 23) The method according to claim 23, wherein the sugar is added to the manure after adjusting a humidity percentage of the manure in a reactor. ### Claim 31 (depends on 30) The method according to claim 30 further comprises the steps of: removing the mannitol from the process liquid; leading a residue of the process liquid into a container; and adjusting the pH value and temperature of the process liquid in the container for production of propionic acid. ### Claim 32 (depends on 31) The method according to claim 31 further comprises the step of adjusting microbial levels of the process liquid using microbial inoculums that is inoculated from a inoculation fermentor, wherein the inoculation fermentor has a dosing pump. ### Claim 33 (depends on 1) The method according to claim 1 further comprises the step of precipitating ammonium salts formed from the manure in the process liquid to fertilizers. ### Claim 34 An apparatus for utilizing intestinal bacteria, said apparatus comprising: a reactor having a configuration capable of containing a process liquid including manure and a sugar, and adjusting a humidity percentage of the manure in the reactor; a container having a configuration capable of receiving a residue of the process liquid from the reactor, and adjusting a pH value and temperature of the residue suitable for production of propionic acid; and an inoculation fermentor having a dosing pump, wherein the inoculation fermentor having a configuration capable of inoculating microbial inoculums therein, and wherein the dosing pump has a configuration capable of pumping the microbial inoculums to the reactor.