# US 2016/0249609 A1 · Insect control sheet > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2016/0249609 A1 - Document ID: US20160249609A1 - Status: Published application - Kind: A1 - Application number: 15/026,855 - Filed: 2015-02-12 - Published: 2016-09-01 - Earliest claimed: 2014-02-18 - Assignees: Nissha Printing Co., Ltd.; National University Corporation Kyoto Institute of Technology - Applicants: Nissha Printing Co., Ltd.; National University Corporation Kyoto Institute of Technology - Inventors: Tetsuya NAKAYAMA; Yasuisa TAKINISHI; Yoshihide INAKO; Hajime MORI; Tomoko HIRANO; Yoshihiro HARADA - Web page: https://patentgrep.com/patent/US20160249609A1 ## CPC - A01N 63/50: Isolated enzymes; Isolated proteins (peptides A01N37/46) - A01M 1/2016: {for flying insects} - A01M 1/2055: {Holders or dispensers for solid, gelified or impregnated insecticide, e.g. volatile blocks or impregnated pads} - A01N 25/34: Shaped forms, e.g. sheets, not provided for in any other sub-group of this main group - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - A01M: CATCHING, TRAPPING OR SCARING OF ANIMALS (appliances for catching swarms or drone-catching A01K57/00; fishing A01K69/00 - {A01K99/00}; biocides, pest repellants or attractants A01N); APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS ## Abstract An insect control sheet containing a Cry polyhedron prepared by fixing an insecticidal protein (a Cry toxin) produced by Bacillus thuringiensis to a polyhedron of polyhedrin protein is provided. The insect control sheet contains the Cry polyhedron and is used by floating on water. The insect control sheet is floatable on water, and includes a pure matrix layer 20 and a toxin-containing matrix layer 30 containing the Cry polyhedron 51 which are layered on the underside of a sheet-shaped first sheet substrate 10. The pure matrix layer 20 is composed of a degradable or water-soluble second material and the toxin-containing matrix layer 30 is composed of a degradable or water-soluble third material and the Cry polyhedron. The toxin-containing matrix layer sustainably releases the Cry polyhedron to the water on which the insect control sheet is floated. ## Claims ### Claim 1 An insect control sheet floating on water, comprising: a sheet-shaped first sheet substrate, a pure matrix layer layered underside the first sheet substrate; a toxin-containing matrix layer layered underside the pure matrix layer; a floating member for floating the insect control sheet on water contained in one of the first substrate, pure matrix layer and the toxin-containing matrix layer, wherein the pure matrix layer comprises a degradable or water-soluble second material, the toxin-containing matrix layer comprises a degradable or water-soluble third material and Cry polyhedron that is an insecticidal protein produced by Bacillus thuringiensis fixes to a polyheadron of polyhedron protein, and the toxin-containing matrix layer releases the Cry polyhedron by decomposition or dissolution of the third material, to the water on which the insect control sheet is floated. ### Claim 2 (depends on 1) The insect control sheet according to claim 1, wherein the second material is composed of the same ingredients as that of the third material. ### Claim 3 (depends on 1) The insect control sheet according to claim 1, wherein the third material comprises at least one ingredient selected from the group consisting of collagen, gelatin, chitin, chitosan, glycosaminoglycan, hyaluronic acid, chondroitin sulfate, elastin, fibronectin, laminin, fibrin, alginic acid, fibroin, starch, pectin, pectic acid, agarose, heparin, carboxymethyl cellulose, cellouronic acid, polyvinyl alcohol, polyethylene glycol, methyl polymethacrylate, methacrylate ester polymers, silicone resins, polylactic acid, polyglycolic acid, polye-caprolactone, bovine serum albumin, casein, sucrose, and mixtures, polymers, copolymers and cross-linked products of those substances. ### Claim 4 (depends on 1) The insect control sheet according to claim 1, wherein the first sheet substrate comprises a degradable first material, and the toxin-containing matrix layer and the pure matrix layer degrade or dissolve in water in a shorter time than that required for the decomposition or dissolution of the first sheet substrate. ### Claim 5 (depends on 1) The insect control sheet according to claim 1, wherein the first sheet substrate comprises nonwoven fabric or mesh sheet. ### Claim 6 (depends on 1) The insect control sheet according to claim 1, wherein the floating member is bubbles contained in the first sheet substrate. ### Claim 7 (depends on 1) The insect control sheet according to claim 1, further comprising a sheet-shaped second sheet substrate that is layered on a top side of the first sheet substrate, wherein the floating member is bubbles contained in the second sheet substrate. ### Claim 8 (depends on 1) The insect control sheet according to claim 1, wherein the floating member is bubbles contained in the toxin-containing matrix layer. ### Claim 9 (depends on 1) The insect control sheet according to claim 1, wherein the first sheet substrate comprises two layers, and the floating member is an air cell being formed between the two layers of the first sheet substrate. ### Claim 10 (depends on 1) The insect control sheet according to claim 1, wherein the floating means is a patterned hydrophobic region being formed on the underside of the first sheet substrate, and the pure matrix layer and toxin-containing matrix layer are layered on the part of the underside of the first sheet substrate where the hydrophobic region is not formed. ### Claim 11 (depends on 1) The insect control sheet according to claim 1, wherein the floating member is a hydrophobic region being formed on the top side of the first sheet substrate. ### Claim 12 (depends on 1) The insect control sheet according to claim 1, wherein a design is made on an underside of the first sheet substrate. ### Claim 13 An insect control article floating on water, comprising: a body floatable on water, a pure matrix layer layered underside the body, a toxin-containing matrix layer layered underside the pure matrix layer; wherein the pure matrix layer comprises a degradable or water-soluble first material; the toxin-containing matrix layer comprises a degradable or water-soluble second material and Cry polyhedron that is an insecticidal protein made of Bacillus thuringiensis fixed to a polyheadron of polyhedron protein; and the toxin-containing matrix layer releases the Cry polyhedron by decomposition or dissolution of the second material to the water on which the insect control article floated. ### Claim 14 A transfer sheet for an insect control sheet or insect control article, comprising: a toxin-containing matrix layer, a pure matrix layer, and an adhesive layer layered in an order on one surface of a transfer-sheet substrate; and wherein the pure matrix layer comprises a degradable or water soluble first material, the toxin-containing matrix layer comprises a degradable or water-soluble second material and a Cry polyhedron that is an insecticidal protein made of Bacillus thuringiensis fixes to a polyhedron of polyhedron protein.