Acoustically ejecting a droplet of fluid from a reservoir by an acoustic fluid ejection apparatus
AssigneeLabcyte Inc.
InventorsRichard G. STEARNS, Shehrzad Ahmed QURESHI
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AssigneeLabcyte Inc.
InventorsRichard G. STEARNS, Shehrzad Ahmed QURESHI
Estimating the term…
The invention provides apparatuses and methods for acoustically ejecting the fluid from a reservoir contained in or disposed on a substrate. The reservoir has a portion adapted to contain a fluid, and an acoustic radiation generator is positioned in acoustic coupling relationship to the reservoir. Acoustic radiation generated by the acoustic radiation generator is transmitted through at least the portion of the reservoir to an analyzer. The analyzer is capable of determining the energy level of the transmitted acoustic radiation and raising the energy level of subsequent pulses to a level sufficient to eject fluid droplets from the reservoir. The invention is particularly suited for delivering fluid from a plurality of reservoirs in an accurate and efficient manner.
-53. (canceled)
A method for characterizing a plurality of fluid reservoirs contained in or located on a substrate by (a) providing a determinable volume fluid having a known composition in each reservoir and adjusting the fluid volume in each reservoir to conform the reservoir to a predetermined ejection threshold level, (b) acoustically coupling a first reservoir to an acoustic ejector that produces acoustic radiation, (c) activating the ejector to generate a sub-threshold pulse of acoustic radiation through the substrate and into the fluid, wherein the sub-threshold pulse's acoustic energy lies below the level effective to eject a droplet from the first reservoir, (d) analyzing the effect of the sub-threshold pulse to determine the gap to the ejection threshold, (e) comparing the ejection threshold with the predetermined ejection threshold, (f) repeating (a), (b), (c), (d) and (e) with each of the plurality of fluid reservoirs in succession, and:
A method for analysis of acoustic data to determine the amount of acoustic energy necessary to increase a perturbation pulse to the level of a droplet ejection pulse, the method comprising the following steps:
A method for analysis of acoustic data to determine the amount of acoustic energy necessary to increase a pulse to an acoustic energy level sufficient for droplet ejection, the method comprising the steps of:
A method for the analysis of acoustic data to determine the amount of additional acoustic energy necessary to change a pulse to the level of a droplet-forming pulse for a fluid surface in a well containing a fluid, the method comprising the steps of:
Dependencies are inferred from the claim text.