Imaging Beta-Amyloid Peptides Aggregation

A method for imaging and detection of beta-amyloid (Aβ) peptides aggregation via magnetic resonance imaging (MRI) based on modified carbazole-based fluorophores comprising a formula S series:

• said method comprising:
• conjugating said carbazole-based fluorophores to magnetic nanoparticles to form a conjugate of said carbazole-based fluorophores and said magnetic nanoparticles;
• introducing said conjugate to a subject with beta-amyloid (Aβ) peptides aggregation;
• and
• applying magnetic resonance imaging (MRI) to image and detect said conjugate bound to the beta-amyloid (Aβ) peptides aggregation in said subject;
• wherein Ar is a heteraromatic ring selected from the group consisting of pyridinyl, substituted pyridinyl, quinolinyl, substituted quinolinyl, acridinyl, substituted acridinyl, benzothiazolyl, substituted benzothiazolyl, benzoxazolyl, and substituted benzoxazolyl;
• R1 is a radical selected from the group consisting of polyethylene glycol chain, alkyl, substituted alkyl, peptide chain, glycosidyl, and C(O)NHCH((CH2CH2O)2CH3)2;
• R2 is selected from the group consisting of ethenyl, ethynyl, azo and azomethinyl.
• R3 is a radical selected from the group consisting of alkyl, HO-alkyl, HS-alkyl, H2N-alkyl, HNalkyl-alkyl, alkyl-COOalkyl, alkyl-CONH2, alkyl-CONHalkyl, alkyl-COOH, alkyl-COO−, (alkyl)3N+-alkyl, and (Ph)3P+-alkyl and polyethylene glycol chain;
• X is an anion selected from the group consisting of F, Cl, Br, I, HSO4, H2PO4, HCO3, tosylate, and mesylate;
• Y is selected from the group consisting of H, F, Cl, OH, OCH3, and R2—Ar—R3.