Theranostic Nanoprobes for Overcoming Cancer Multidrug Resistance and Methods
AssigneeMassachusetts Institute of Technology
InventorsNatalie Artzi, João Conde, Nuria Oliva
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Abstract
Theranostic nanoprobes are provided for overcoming cancer multidrug resistance, and methods for treating biological tissue, including cancerous tissue. The theranostic nanoprobes may include gold nanoparticles functionalized with DNA-hairpin. The DNA-hairpin may be configured to hybridize to a complementary target, which may silence or lessen the multidrug resistance of cancer cells. The theranostic nanoprobes may be configured to release a chemotherapeutic agent upon hybridization of the DNA-hairpin to a target molecule.
Claims33
A theranostic nanoprobe comprising:
- a gold nanoparticle functionalized with
- at least one DNA-hairpin labeled with a fluorophore, and
- at least one anchor labeled with a quencher;
- and
- a chemotherapeutic agent intercalated in the at least one DNA-hairpin;
- wherein the at least one DNA-hairpin is configured to hybridize to a complementary target in a cancer cell.
A method for treating a biological tissue, the method comprising:
- providing a hydrogel comprising an embedded theranostic nanoprobe;
- and
- contacting the biological tissue with the hydrogel;
- wherein the embedded theranostic nanoprobe comprises
- a gold nanoparticle functionalized with
- at least one DNA-hairpin labeled with a fluorophore, and
- at least one anchor labeled with a quencher;
- and
- a chemotherapeutic agent intercalated in the at least one DNA-hairpin;
- wherein the at least one DNA-hairpin is configured to hybridize to a complementary target in a cancer cell.
A method for treating a biological tissue, the method comprising:
- providing a first solution comprising a polymer component, wherein the polymer component comprises a polymer having three or more aldehyde groups;
- providing a second solution comprising a dendrimer component, wherein the dendrimer component comprises a dendrimer having at least 2 branches with one or more surface groups;
- wherein at least one of the first solution and the second solution comprises a theranostic nanoprobe;
- and
- combining the first and second solutions together to produce a hydrogel and contacting one or more biological tissues with the hydrogel;
- wherein the embedded theranostic nanoprobe comprises
- a gold nanoparticle functionalized with
- at least one DNA-hairpin labeled with a fluorophore, and
- at least one anchor labeled with a quencher;
- and
- a chemotherapeutic agent intercalated in the at least one DNA-hairpin;
- wherein the at least one DNA-hairpin is configured to hybridize to a complementary target in a cancer cell.
A hydrogel composition comprising:
- a plurality of theranostic nanoprobes, each of which comprises
- a gold nanoparticle functionalized with
- at least one DNA-hairpin labeled with a fluorophore, and
- at least one anchor labeled with a quencher;
- and
- a chemotherapeutic agent intercalated in the at least one DNA-hairpin,
- wherein the at least one DNA-hairpin is configured to hybridize to a complementary target in a cancer cell;
- and
- a biocompatible hydrogel in which the plurality of theranostic nanoprobes are dispersed.
A kit comprising:
- a first solution comprising a polymer component, wherein the polymer component comprises a polymer having three or more aldehyde groups;
- a second solution comprising a dendrimer component, wherein the dendrimer component comprises a dendrimer having at least 2 branches with one or more surface groups;
- and
- a theranostic nanoprobe dispersed in either the first solution, the second solution, or both, wherein the theranostic nanoprobe comprises
- a gold nanoparticle functionalized with
- at least one DNA-hairpin labeled with a fluorophore, and
- at least one anchor labeled with a quencher;
- and
- a chemotherapeutic agent intercalated in the at least one DNA-hairpin;
- wherein the at least one DNA-hairpin is configured to hybridize to a complementary target in a cancer cell.
A theranostic nanoprobe comprising:
- a gold nanoparticle functionalized with at least one DNA-hairpin;
- and
- a chemotherapeutic agent intercalated in the at least one DNA-hairpin;
- wherein the at least one DNA-hairpin is configured to hybridize to a complementary target in a cancer cell.
Dependencies are inferred from the claim text.