Compositions and methods comprising a defined microbiome and methods of use thereof
AssigneeThe Trustees of the University of Pennsylvania
InventorsFrederic Bushman, Gary Wu, James Lewis, Mark Goulian
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Abstract
The invention features the use of a defined microbial consortia for the replacement of a gut microbiome associated with disease. In particular, the invention provides for the treatment of hyperammonemia, Clostridium difficile colitis, hepatic encephalopathy associated with cirrhosis, and inflammatory bowel disease.
Claims28
A method for altering gut microbiota to reduce urease gene content or activity via reducing or eliminating urease producing bacteria from the gut in order to reduce ammonia production therefrom, in a subject in need thereof, the method comprising;
- administering to the subject a composition comprising a defined microbial consortia of bacteria having minimal urease activity or no urease gene content, under conditions wherein said bacteria colonize the gut, thereby reducing urease gene content, or activity or both in gut microbiota and ammonia production in the gut of the subject.
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A composition comprising an effective amount of a defined microbial consortia comprising a combination of one or more bacteria selected from the group consisting of Bifidobacterium, Collinsella, Bacteroides, Petrimonas, Tannerella, Parabacteroides, Bacillus, Isobaculum, Enterococcus, Lactobacillus, Anaerostipes, Blautia, Coprococcus, Dorea, Oribacterium, Syntrophococcus, Clostridium XI, Catenibacterium, Mitsuokella, Escherichia/Shigella, and Parapervotella, said consortia being effective to reduce urease levels in gut microbiota, said composition being present in a biological carrier suitable for administration to, and colonization, of the gut.
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A method for altering gut microbiota to reduce urease gene content or activity via reducing or eliminating urease producing bacteria from the gut in order to reduce ammonia production therefrom, in a subject in need thereof, the method comprising;
- administering to the subject a composition comprising a single bacteria species lacking urease gene content or activity, under conditions wherein said bacteria colonizes the gut, thereby reducing urease gene content, or activity or both in gut microbiota and ammonia production in the gut of the subject.
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Dependencies are inferred from the claim text.