Dual vector for inhibition of human immunodeficiency virus
AssigneeCalimmune, Inc.
InventorsIrvin CHEN, Dong Sung AN, Michelle MILLINGTON, Maureen BOYD, Geoffrey P. SYMONDS, Louis Randall BRETON
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Abstract
The present invention provides an expression vector for preventing or inhibiting HIV entry, fusion or replication in mammalian cells. In particular, the invention provides a recombinant retroviral vector that encodes an inhibitor of a HIV co-receptor, such as CCR5 or CXCR4, and a protein that inhibits HIV fusion to target cells and/or HIV replication. Pharmaceutical compositions comprising such constructs and methods of use thereof to prevent or treat HIV infection in a patient are also disclosed.
Claims25
(canceled)
A method of treating or preventing HIV infection in a patient comprising (i) transducing hematopoietic cells with an expression vector, the expression vector comprising a first nucleic acid sequence encoding an inhibitor of an HIV co-receptor and a second nucleic acid sequence encoding a protein that inhibits HIV fusion to a target cell or HIV replication, and (ii) transplanting said transduced hematopoietic cells in the patient, wherein said transduced hematopoietic cells are resistant to HIV infection.
The method of claim 4, wherein said transplanted HPSC generate granulocytes, monocyte/macrophages, and lymphocytes that are resistant to HIV infection.
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14-17. (canceled)
A method of treating or preventing HIV infection in a patient comprising administering a pharmaceutical composition to patient, the pharmaceutical composition comprising (i) an expression vector comprising a first nucleic acid sequence encoding an inhibitor of an HIV co-receptor and a second nucleic acid sequence encoding a protein that inhibits HIV fusion to a target cell or HIV replication;
- and (ii) a pharmaceutically acceptable carrier.
21-24. (canceled)
A method of treating or preventing HIV infection in a patient comprising:
- (i) transducing hematopoietic cells with an expression vector, the expression vector comprising a first nucleic acid sequence encoding a shRNA having a sequence of SEQ ID NO:
- 1, and a second nucleic acid sequence encoding a C46 protein, and
- (ii) transplanting said transduced hematopoietic cells in the patient, wherein said transduced hematopoietic cells are resistant to HIV infection.
Dependencies are inferred from the claim text.