Microfluidic Assay Devices And Methods For Making And Using The Same
AssigneeThe Regents of the University of California
InventorsAmy E. Herr, Robert Lin
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Abstract
Microfluidic devices for determining whether an analyte is present in a sample are provided. The microfluidic devices include a polymeric medium that includes a first analyte detection domain having a first covalently bound capture member that specifically binds to a first analyte, and a second analyte detection domain having a second covalently bound capture member that specifically binds to a second analyte. Also provided are methods of using the subject microfluidic device, systems and kits that use the subject microfluidic devices, as well as methods of producing the same.
Claims25
A microfluidic device comprising:
- a polymeric medium comprising:
- a first analyte detection domain comprising a first covalently bound capture member that specifically binds to a first analyte;
- and
- a second analyte detection domain comprising a second covalently bound capture member that specifically binds to a second analyte.
A method of determining whether an analyte is present in a sample, the method comprising:
- (a) introducing a sample into a microfluidic device comprising a polymeric medium, wherein the polymeric medium comprises:
- (i) a first analyte detection domain comprising a first covalently bound capture member that specifically binds to a first analyte;
- and
- (ii) a second analyte detection domain comprising a second covalently bound capture member that specifically binds to a second analyte;
- (b) applying a directional electric field to the polymeric medium in a manner sufficient to move components in the sample through the polymeric medium;
- and
- (c) obtaining a signal from one or more of the first and second analyte detection domains to determine whether the analyte is present in the sample.
A method of producing a microfluidic assay device, the method comprising:
- producing a polymeric medium comprising functional groups that covalently bond to a capture member upon application of an applied stimulus;
- introducing into the polymeric medium a first capture member that specifically binds to a first analyte;
- exposing a first region of the polymeric medium to the applied stimulus to produce a first analyte detection domain that comprises the first capture member covalently bound to the polymeric medium;
- introducing into the polymeric medium a second capture member that specifically binds to a second analyte;
- and
- exposing a second region of the polymeric medium to the applied stimulus to produce a second analyte detection domain that comprises the second capture member covalently bound to the polymeric medium,
- to produce the microfluidic assay device.
Dependencies are inferred from the claim text.