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US 2016/0238511 A1Published application
Optical detection systems and methods of using the same
AssigneeBecton, Dickinson and Company
InventorJianying Cao
Life of this patent
Estimating the term…
Abstract
Optical detection systems configured to collect and detect light emitted by a sample are provided. Aspects of the systems include a core region selective side scatter (SSC) collection module configured to allow for the selective detection SSC wavelength light from a core region of collected light. Also provided are methods of using the systems.
Claims23
An optical detection system comprising:
- a light collection system configured to produce objective lens imaged collected light from a detection zone of a flow cell;
- a light dispersion module configured to separate the objective lens imaged collected light into at least two wavelengths of light;
- a first detector configured to detect a first of the at least two wavelengths of light and a second detector configured to detect a second of the at least two wavelengths of light, wherein the first wavelength of light is SSC wavelength light;
- and
- an aperture positioned between the light collection system and the light dispersion module, wherein the aperture comprises an optical mask configured to selectively block SSC wavelength light from the objective lens imaged collected light that is outside of a core region from reaching the first detector.
2 more elements
(canceled)
An optical detection system comprising:
- a light collection system configured to produce objective lens imaged collected light from a detection zone of a flow cell;
- a detector configured to receive the objective lens imaged collected SSC wavelength light;
- an optical filter configured to produce at least a first path and a second path of objective lens imaged collected light, wherein the first path comprises at least a portion of the SSC wavelength light from the objective lens imaged collected light, and wherein the first path is transmitted to the detector;
- and
- an aperture configured to block passage of SSC wavelength light in the first path that is outside of a core region from reaching the detector.
2 more elements
An optical detection system comprising:
- a light collection system configured to produce objective lens imaged collected light from a detection zone of a flow cell;
- a detector configured to receive the objective lens imaged collected SSC wavelength light;
- an optical filter configured to produce at least a first path and a second path of objective lens imaged collected light, wherein the first path comprises at least a portion of the SSC wavelength light from the objective lens imaged collected light, and wherein the first path is transmitted to the detector;
- and
- a fiber optic light conveyer configured to selectively transmit SSC wavelength light in the first path from a core region of the SSC wavelength light to the detector.
2 more elements
21-29. (canceled)
A method of analyzing a flow stream, the method comprising:
- irradiating a flow stream with a light source;
- generating objective lens imaged light collected from a detection zone of the irradiated flow stream;
- and
- passing the objective lens imaged collected light through an aperture to a light dispersion module, wherein:
- (i) the aperture comprises an optical mask that selectively blocks side scatter (SSC) wavelength light outside of a core region from passing through;
- and
- (ii) the light dispersion module is configured to separate the objective lens imaged collected light into at least a first and a second wavelength of light, wherein the first wavelength of light is SSC wavelength light;
- and
- detecting the first and second wavelengths of light to analyze the flow stream.
6 more elements
31-56. (canceled)
Dependencies are inferred from the claim text.