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US 2016/0237484 A1Published application
Method and composition for nucleic acid amplification
AssigneeApplied Biosystems, LLC
InventorsJULIO J. MULERO, Lori K. Hennessy
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Abstract
The present teachings provide methods, compositions, and kits for nucleic acid amplification. In some embodiments of the present teachings, amplification reactions are performed with at least one high stability primer. In some embodiments, the present teachings provide a method comprising a high stability primer for amplification of a nucleic acid sequence in a sample comprising a target nucleic acid sequence and a PCR inhibitor.
Claims12
-49. (canceled)
A method comprising:
- contacting a sample comprising a nucleic acid and a PCR inhibitor with a primer, wherein the PCR inhibitor comprises humic acid, wherein the primer comprises one high stability nucleic acid analog, wherein the one high stability nucleic acid analog comprises an LNA;
- and
- performing a PCR amplification reaction on the sample, wherein the primer comprising one LNA exhibits a higher degree of amplification in the presence of humic acid as compared to a standard stability primer not comprising LNA which possesses the same nucleotide sequence as the primer comprising LNA.
Dependencies are inferred from the claim text.