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US 2016/0237483 A1Published application
Helicase Suppression of Non-Template Amplification
AssigneeNew England Biolabs, Inc.
InventorsNathan Tanner, Thomas C. Evans, JR.
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Abstract
Provided herein is a method for reducing amplification of non-template molecules in a nucleic acid sample. In certain embodiments, the method involves adding a helicase to a reaction mixture for non-helicase-dependent amplification of target nucleic acid.
Claims20
A reaction mixture comprising:
- a) a nucleic acid sample comprising a template;
- b) nucleotides;
- c) four or more primers;
- d) a polymerase;
- and
- e) a helicase;
- wherein the reaction mixture does not contain a single-stranded DNA binding protein (SSBP) and wherein the reaction mixture is capable of amplifying the template when placed under isothermal or polymerase chain reaction conditions.
4 more elements
A method for reducing amplification of non-template molecules from a nucleic acid sample, comprising:
- a) incubating a reaction mixture comprising a nucleic acid sample comprising a template, nucleotides, at least four primers, a polymerase, and a helicase under amplification conditions, and
- b) amplifying the template;
- wherein the amplification reaction is not helicase dependent but wherein the helicase reduces amplification of non-template molecules.
Dependencies are inferred from the claim text.