Methods for biosynthesizing methacrylate
AssigneeInvista Technologies S.A.R.L.
InventorsAdriana Leonora Botes, Alex Van Eck Conradie
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Abstract
This document describes biochemical pathways for producing methacrylate from precursors such as pyruvate via isobutyraldehyde and isobutyryl-CoA, using enzymes such as one or more thioesterases, transferases, or dehydrogenases, as well as recombinant hosts expressing one or more of such enzymes.
Claims33
A method of producing methacrylate, said method comprising enzymatically synthesizing methacryloyl-CoA from pyruvate via isobutyraldehyde, and enzymatically converting methacryloyl-CoA to methacrylate using a CoA-transferase.
The method of 13, wherein the host is a prokaryote or a eukaryote.
A recombinant host comprising at least one exogenous nucleic acid encoding a phenylacetaldehyde dehydrogenase, wherein said recombinant host produces methacrylic acid.
A non-naturally occurring biochemical network comprising at least one substrate of FIG. 1, at least one exogenous nucleic acid encoding a polypeptide having the activity of at least one enzyme of FIG. 1 and at least one product of FIG. 1.
A non-naturally occurring composition, comprising at least one substrate of FIG. 1, at least one exogenous nucleic acid encoding a polypeptide having the activity of at least one enzyme of FIG. 1 and at least one bio-derived product of FIG. 1.
Dependencies are inferred from the claim text.