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US 2016/0237448 A1Published application
Novel acyltranserases and methods of using
AssigneeNUtech Ventures
InventorsEdgar Cahoon, Umidjon Iskandarov, Hae Jin Kim, Jillian Collins-Silva
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Abstract
Provided herein are novel acyltransferases and methods of using such novel acyltransferases in making medium-chain fatty acids.
Claims20
A method of producing triacylglycerols (TAGs) comprising medium-chain fatty acids (MCFAs) in an organism, comprising:
- introducing a transgene into the organism, wherein the transgene comprises at least one nucleic acid sequence encoding an acyltransferase, wherein the at least one acyltransferase exhibits a substrate specificity for saturated fatty acids;
- thereby producing TAGs comprising MCFAs in the organism.
A method of producing triacylglycerols (TAGs) comprising medium-chain fatty acids (MCFAs), comprising:
- providing an organism comprising a transgene, wherein the transgene comprises at least one nucleic acid sequence encoding an acyltransferase, wherein the at least one acyltransferase exhibits a substrate specificity for saturated fatty acids;
- growing the organism under appropriate conditions;
- and
- obtaining TAGs comprising MCGAs from the organism.
1 more element
A method of increasing the amount of triacylglycerols (TAGs) comprising medium-chain fatty acids (MCFAs) in the seed oil of a plant, comprising:
- providing a plant comprising a nucleic acid encoding a FatB polypeptide;
- introducing a heterologous nucleic acid molecule into the plant comprising at least one nucleic acid sequence encoding an acyltransferase, wherein the at least one acyltransferase exhibits a substrate specificity for saturated fatty acids,
- thereby increasing the amount of TAGs comprising MCFAs in the seed oil of the plant without significantly changing the total oil content in the seed.
Dependencies are inferred from the claim text.