Transgenic Plants With Enhanced Traits

A method for producing a plant comprising:

• introducing into a plant cell a recombinant DNA molecule comprising a polynucleotide encoding a polypeptide, wherein the polynucleotide comprises a polynucleotide sequence selected from the group consisting of:
• a) a nucleotide sequence as set forth as SEQ ID NO:
• 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57 or 115;
• b) a nucleotide sequence encoding a protein comprising an amino acid sequence as set forth in SEQ ID NO:
• 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 59-92 or 116;
• c) a nucleotide sequence with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% identity to SEQ ID NO:
• 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57 or 115;
• and
• d) a nucleotide sequence encoding a protein with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% identity to SEQ ID NO:
• 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 59-92 or 116;
• and growing a plant from said plant cell.

A recombinant DNA molecule, said molecule comprising a promoter operably linked to a polynucleotide that is transcribed into a non-coding miRNA molecule, wherein:

• a) said non-coding miRNA molecule is selected from the group consisting of:
• i) a non-coding miRNA molecule which, when present in a plant, provides an altered level of a protein as compared to a control plant, wherein said protein is selected from the group consisting of:
• a phosphate transporter protein, an APETALA2-like transcription factor, an ANR1 MADS-box protein, an E3 ligase SCF complex F-box protein, a HOS1 protein, a bHLH transcription factor, a diphenol oxidase proteinligase;
• ii) a non-coding miRNA molecule which, when present in a plant, provides an altered level of a protein as compared to a control plant, wherein said protein is selected from the group consisting of:
• the polypeptide sequences set forth in SEQ ID NO:
• 108, 110, 112 and 114;
• iii) a non-coding miRNA molecule which, when present in a plant, interferes with the functioning of one or more species of an endogenous miRNA selected from the group consisting of:
• miR399, miR172, miR166, miR166, miR444, miR393 and miR397;
• iv) a non-coding miRNA molecule comprising a miRNA binding site sequence as set forth in SEQ ID NOs:
• 100-106;
• v) a non-coding miRNA molecule comprising a miRNA polynucleotide sequence as set forth in SEQ ID NOs:
• 93-99;
• and
• b) said promoter is selected from the group consisting of a constitutive promoter, a developmental promoter, a tissue enhanced promoter, a tissue preferred promoter, a tissue specific promoter, a cell type-specific promoter, an inducible promoter and a diurnal promoter;
• wherein, when said recombinant DNA molecule is present in a plant, said plant exhibits an altered phenotype or an enhanced trait as compared to a control plant.

A plant comprising a recombinant DNA molecule, said molecule comprising a heterologous promoter functional in plant cells operably linked to a polynucleotide that is transcribed into a non-coding miRNA molecule, wherein

• a) said non-coding miRNA molecule is selected from the group consisting of:
• i) a non-coding miRNA molecule which, when present in a plant, provides an altered level of a protein as compared to a control plant, wherein said protein is selected from the group consisting of:
• a phosphate transporter protein, an APETALA2-like transcription factor, an ANR1 MADS-box protein, an E3 ligase SCF complex F-box protein, a HOS1 protein, a bHLH transcription factor, a diphenol oxidase proteinligase;
• ii) a non-coding miRNA molecule which, when present in a plant, provides an altered level of a protein as compared to a control plant, wherein said protein is selected from the group consisting of:
• the polypeptide sequences set forth in SEQ ID NO:
• 108, 110, 112 and 114;
• iii) a non-coding miRNA molecule which, when present in a plant, interferes with the functioning of one or more species of an endogenous miRNA selected from the group consisting of:
• miR399, miR172, miR166, miR166, miR444, miR393 and miR397;
• iv) a non-coding miRNA molecule comprising a miRNA binding site sequence as set forth in SEQ ID NOs:
• 100-106;
• v) a non-coding miRNA molecule comprising a miRNA polynucleotide sequence as set forth in SEQ ID NOs:
• 93-99;
• and
• b) said promoter is selected from the group consisting of a constitutive promoter, a developmental promoter, a tissue enhanced promoter, a tissue preferred promoter, a tissue specific promoter, a cell type-specific promoter, an inducible promoter and a diurnal promoter;
• wherein said plant has an enhanced trait as compared to a control plant.

A method for producing a plant comprising:

• introducing into a plant cell a recombinant DNA molecule comprising a heterologous promoter functional in plant cells operably linked to a polynucleotide that is transcribed into a non-coding miRNA molecule, wherein
• a) said plants exhibit an altered level of a protein selected from the group consisting of:
• a phosphate transporter protein, an APETALA2-like transcription factor, an ANR1 MADS-box protein, an E3 ligase SCF complex F-box protein, a HOS1 protein, a bHLH transcription factor, a diphenol oxidase proteinligase;
• or
• b) said plants exhibit an altered level of a protein selected from the group consisting of:
• the polypeptides set forth in SEQ ID NO:
• 108, 110, 112 and 114;
• or
• c) said non-coding miRNA molecule interferes with the functioning of one or more species of an endogenous miRNA selected from the group consisting of:
• miR399, miR172, miR166, miR166, miR444, miR393 and miR397;
• or
• d) said non-coding miRNA molecule comprises a miRNA binding site polynucleotide sequence as set forth in SEQ ID NOs:
• 100-106;
• or
• e) said non-coding miRNA molecule comprises a miRNA polynucleotide sequence as set forth in SEQ ID NOs:
• 93-99;
• and
• growing a plant from said plant cell.