Zea mays regulatory elements and uses thereof
AssigneeDow AgroSciences LLC
InventorsManju Gupta, Sara Bennett, Navin Elango, Karthik Muthuraman, Jeffrey Beringer, Huixia Wu
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Abstract
Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using Zea mays chlorophyll a/b binding gene regulatory elements.
Claims23
A gene expression cassette comprising a promoter operably linked to a transgene, wherein the promoter comprises a polynucleotide that hybridizes under stringent conditions to a polynucleotide probe comprising a sequence identity of at least 90% to a complement of SEQ ID NO:2.
A method for expressing a heterologous coding sequence in a transgenic plant, the method comprising:
- a) transforming a plant cell with a gene expression cassette comprising a polynucleotide sequence comprising a sequence identity of at least 90% to SEQ ID NO:2 operably linked to the heterologous coding sequence, which is operably linked to a 3′-untranslated region;
- b) isolating the transformed plant cell comprising the gene expression cassette;
- c) regenerating the transformed plant cell into a transgenic plant;
- and,
- d) obtaining the transgenic plant, wherein the transgenic plant comprises the gene expression cassette comprising the polynucleotide sequence comprising SEQ ID NO:2.
A method for manufacturing a synthetic polynucleotide sequence comprising a sequence identity of at least 90% to SEQ ID NO:2, the method comprising:
- a) identifying the polynucleotide sequence comprising SEQ ID NO:2;
- b) isolating the polynucleotide sequence comprising SEQ ID NO:2;
- c) defining a plurality of polynucleotide sequences that comprise a sequence identity of at least 90% to SEQ ID NO:2;
- d) synthesizing a polynucleotide sequence comprising a sequence identity of at least 90% to SEQ ID NO:2;
- and,
- e) manufacturing a synthetic polynucleotide sequence comprising a sequence identity of at least 90% to SEQ ID NO:2.
Dependencies are inferred from the claim text.