Modified group i methanotrophic bacteria and uses thereof
AssigneeUniversity of Washington through its Center for Commercialization
InventorsAaron Puri, Mary E. Lidstrom, Marina Kalyuzhnaya
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Abstract
Described herein are methods and compositions relating to engineered methanotrophic bacterium and the production of carbon products from methane.
Claims29
An engineered methanotrophic bacterium, the bacterium comprising a genetic alteration causing a modulation selected from the group consisting of:
- an increase in the conversion of methane to pyruvate and/or AcCoA;
- a decrease in the activity of a pathway that diverts formate and/or pyruvate from fatty acid biosynthesis;
- a decrease in lipid degradation activity;
- and
- an increase in fatty ester production.
An engineered methanotrophic bacterium, the bacterium comprising a genetic alteration which modulates the expression of a gene product as specified in Table 9.
A method of engineering a methanotrophic bacterium to increase pyruvate flux, the method comprising genetically altering a methanotrophic bacterium to cause a modulation selected from the group consisting of:
- an increase in the conversion of methane to pyruvate and/or AcCoA;
- a decrease in the activity of a pathway that diverts formate and/or pyruvate from fatty acid biosynthesis;
- a decrease in lipid degradation activity;
- and
- an increase in fatty ester production.
A method of increasing the flux of carbon from methane to pyruvate, the method comprising treating a methanotrophic bacterium to alter the expression or activity of a gene product as specified in Table 9.
Dependencies are inferred from the claim text.