Engineering of Acetyl-CoA Metabolism in Yeast
AssigneeBiopetrolia AB
InventorsJens Nielsen, Verena Siewers, Anastasia Krivoruchko, Yiming Zhang, Zongjie Dai
Life of this patent
Estimating the term…
Abstract
The invention relates to engineering of acetyl-CoA metabolism in yeast and in particular to production of acetyl-CoA in a non-ethanol producing yeast lacking endogenous gene(s) encoding pyruvate decarboxylase and comprising a heterologous pathway for synthesis of cytosolic acetyl-CoA.
Claims24
1-22. (canceled)
A yeast comprising at least one heterologous pathway for synthesis of cytosolic acetyl-Coenzyme A (CoA), the at least one heterologous pathway comprising at least one heterologous gene encoding a respective enzyme involved in synthesis of acetyl-CoA, with the proviso that the at least one heterologous gene is not a heterologous gene encoding a pyruvate formate lyase, wherein the at least one heterologous gene comprises:
- heterologous genes encoding a cytosolic pyruvate dehydrogenase complex;
- and
- heterologous genes encoding respective enzymes involved in attachment and activation of lipoyl groups to said cytosolic pyruvate dehydrogenase complex.
A yeast comprising at least one heterologous pathway for synthesis of cytosolic acetyl-Coenzyme A (CoA), the at least one heterologous pathway comprising at least one heterologous gene encoding a respective enzyme involved in synthesis of acetyl-CoA, with the proviso that the at least one heterologous gene is not a heterologous gene encoding a pyruvate formate lyase, wherein the at least one heterologous gene comprises:
- a heterologous gene encoding a pyruvate ferredoxin oxidoreductase;
- a heterologous gene encoding a ferredoxin reductase;
- and/or
- a heterologous gene encoding a ferredoxin reductase substrate selected from the group consisting of ferredoxin and flavodoxin.
A method of producing a yeast suitable for the production of cytosolic acetyl-Coenzyme A (CoA) comprising:
- introducing, into said yeast, at least one heterologous pathway for synthesis of cytosolic acetyl-CoA, said at least one heterologous pathway comprises at least one heterologous gene encoding a respective enzyme involved in synthesis of acetyl-CoA, with the proviso that said at least one heterologous gene is not a heterologous gene encoding a pyruvate formate lyase.
- wherein the at least one heterologous gene comprises:
- heterologous genes encoding a cytosolic pyruvate dehydrogenase complex;
- and
- heterologous genes encoding respective enzymes involved in attachment and activation of lipoyl groups to said cytosolic pyruvate dehydrogenase complex;
- or
- a heterologous gene encoding a pyruvate ferredoxin oxidoreductase;
- a heterologous gene encoding a ferredoxin reductase;
- and/or
- a heterologous gene encoding a ferredoxin reductase substrate selected from the group consisting of ferredoxin and flavodoxin.
Dependencies are inferred from the claim text.