Protease variants with increased stability
AssigneeHenkel AG & Co. KGaA
InventorsHendrik Hellmuth, Thomas Weber, Timothy O'Connell
Life of this patent
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Abstract
The invention relates to proteases comprising an amino acid sequence which has an at least 70% sequence identity with the amino acid sequence set forth in SEQ ID NO:1, over the entire length thereof, and in which the amino acids at positions corresponding to positions 3, 4, 99, 188, and 199 according to SEQ ID NO:1 are substituted to S3T, V4I, R99D/E, A188P and V199I, preferably S3T, V4I, R99E, A188P, and V199I, and to the production and use thereof. Such proteases exhibit very good stability, in particular temperature stability, while at the same time having good cleaning power.
Claims10
A protease comprising an amino acid sequence, which has an at least 70% sequence identity with the amino acid sequence set forth in SEQ ID NO:1 over the entire length thereof and which has the amino acid substitutions S3T, V4I, R99D/E, A188P, and V199I based on the numbering according to SEQ ID NO:1.
A method for producing a protease comprising the substitution of the amino acids at positions, corresponding to positions 3, 4, 99, 188, and 199 in SEQ ID NO:1, in an original protease, which has an at least 70% sequence identity with the amino acid sequence set forth in SEQ ID NO:1 over the entire length thereof, in such a way that the protease comprises the amino acids 3T, 4I, 99D/E, 188P, and 199I at the corresponding positions.
Dependencies are inferred from the claim text.