Adipose derived adult stem cells in hepatic regeneration
AssigneesLouisiana State University, Artecel, Inc.
InventorsJeffery M. GIMBLE, John W. LUDLOW
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Abstract
The present invention provides a method to derive hepatic stem cells from stem cells derived from non-liver tissue. In one embodiment of the invention, hepatic stem cells are derived from adipose stem cells. The invention also provides a method of enhancing hepatic cytokine production (e.g., HGF) from ASCs, which may be useful in the regeneration of liver tissue when transplanted in vivo. Tissue culture conditions, including media conditions, are provided.
Claims13
A method of deriving hepatic stem cells from non-liver stem cells comprising:
- (a) providing stem cells derived from non-liver tissue;
- and
- (b) culturing the stem cells on an extracellular matrix comprising at least one fibrilar matrix protein and in serum-free culture medium comprising hepatic growth factor (HGF),
- in which the stem cells derived from non-liver tissue differentiate into hepatic stem cells.
A method of inducing HGF expression from non-liver stem cells comprising:
- (a) providing stem cells derived from non-liver tissue;
- and
- (b) culturing the stem cells on an extracellular matrix comprising at least one fibrilar matrix protein and in serum-free culture medium comprising EGF, bFGF, or both,
- in which the stem cells derived from non-liver tissue produce HGF.
Dependencies are inferred from the claim text.