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US 2016/0237398 A1Published application
Methods of microbial production of excreted products from methane and related bacterial strains
AssigneeUniversity of Washington through its Center for Commercialization
InventorsMarina Kalyuzhnaya, Mary E. Lidstrom
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Abstract
The present disclosure is directed to methods of producing excreted products through the fermentation of methane with methanotrophs. In certain embodiments, the methods are performed at low oxygen levels.
Claims31
A method for producing at least one excreted product by microbial fermentation of a gaseous substrate, comprising:
- a. providing a gaseous substrate comprising CH4 and optionally, O2, to a culture of at least one methanotrophic microorganism;
- and
- b. maintaining the microorganism under conditions suitable for fermentation at a dissolved O2 tension of between 0 and about 1% of saturation with air to produce at least one excreted product;
- or
- maintaining the microorganism under conditions suitable for fermentation at a dissolved O2 tension of between 0 and about 40% of saturation with air and reducing respiration to produce at least one excreted product.
2 more elements
A method for producing at least one excreted product by microbial fermentation of a gaseous substrate, comprising:
- a. providing a gaseous substrate comprising CH4 and optionally, O2, to a culture of at least one methanotrophic microorganism;
- and
- b. maintaining the microorganism under conditions suitable for fermentation to produce at least one excreted product.
An engineered methanotrophic bacterium capable of fermenting methane comprising a deletion of one or more of the following genes:
- a. NADH-ubiquinone oxidoreductase (MALCv4_1304);
- b. hydrogenase (MALCv4_1307);
- c. acetate kinase (MALCv4_2853);
- and
- d. lactate dehydrogenase (MALCv4_0534);
- e. NAD-reducing hydrogenase (MALCv4_1304 and 1307);
- f. bacteriohemelythrin (MALCv4_2316);
- g. sucrose-phosphate synthase (MALCv4_0614);
- h. a member of the cytochrome bc1 complex (MALCv4_0634, MALCv4_0633, and MALCv4_0632);
- i. a glycogen biosynthesis gene (MALCv4_3502;
- MALCv4_3503;
- MALCv_3504;
- MALCv4_3505;
- MALCv_3506;
- MALCv4_3507, and MALCv_3508);
- and
- j. cytochrome aa3 oxidase (MALCv4_2315).
14 more elements
Dependencies are inferred from the claim text.