Methods and devices for breaking cell aggregation and separating or enriching cells
AssigneeAviva Biosciences Corporation
InventorsAntonio Guia, Ky Truong
Life of this patent
Estimating the term…
Abstract
In one aspect, the present disclosure provides a method for separating a target component in a fluid sample, which method comprises: a) passing a fluid sample that comprises or is suspected of comprising a target component and cell aggregates through a microfabricated filter so that said target component, if present in said fluid sample, is retained by or passes through said microfabricated filter, and b) prior to and/or concurrently with passing said fluid sample through said microfabricated filter, contacting said fluid sample with an emulsifying agent to reduce or remove said cell aggregates, if present in said fluid sample.
Claims106
A method for separating a target component in a fluid sample, which method comprises:
- a) passing a fluid sample that comprises or is suspected of comprising a target component and cell aggregates through a microfabricated filter so that said target component, if present in said fluid sample, is retained by or passes through said microfabricated filter, and
- b) prior to and/or concurrently with passing said fluid sample through said microfabricated filter, contacting said fluid sample with an emulsifying agent and/or a cellular membrane charging agent to reduce or disaggregate said cell aggregates, if present in said fluid sample, and/or
- prior to and/or concurrently with passing said fluid sample through said microfabricated filter, contacting said fluid sample with a hyperosmotic saline solution between about 350 mOsm and about 1000 mOsm, optionally between about 400 mOsm and about 600 mOsm, to reduce or disaggregate said cell aggregates, if present in said fluid sample.
A device, system or package for separating a target component in a fluid sample that comprises or is suspected of comprising a target component and cell aggregates, which device, system or package comprises:
- a) a filtration chamber according to any one of embodiments 1-80;
- and
- b) an effective amount of an emulsifying agent and/or a cellular membrane charging agent to reduce or disaggregate said cell aggregates, if present in said fluid sample;
- and/or, a hyperosmotic saline solution between about 350 mOsm and about 1000 mOsm, optionally between about 400 mOsm and about 600 mOsm, to reduce or disaggregate said cell aggregates, if present in said fluid sample.
A device, system or package for separating a target component in a fluid sample that comprises or is suspected of comprising a target component and cell aggregates, which device, system or package comprises:
- a) a cartridge according to any one of embodiments 81-83;
- and
- b) an effective amount of an emulsifying agent and/or a cellular membrane charging agent to reduce or disaggregate said cell aggregates, if present in said fluid sample;
- and/or, a hyperosmotic saline solution between about 350 mOsm and about 1000 mOsm, optionally between about 400 mOsm and about 600 mOsm, to reduce or disaggregate said cell aggregates, if present in said fluid sample.
A device, system or package for separating a target component in a fluid sample that comprises or is suspected of comprising a target component and cell aggregates, which device, system or package comprises:
- a) an automated filtration unit according to any one of embodiments 84-99;
- and
- b) an effective amount of an emulsifying agent and/or a cellular membrane charging agent to reduce or disaggregate said cell aggregates, if present in said fluid sample;
- and/or, a hyperosmotic saline solution between about 350 mOsm and about 1000 mOsm, optionally between about 400 mOsm and about 600 mOsm, to reduce or disaggregate said cell aggregates, if present in said fluid sample.
A system or package for separating a target component in a fluid sample that comprises or is suspected of comprising a target component and cell aggregates, which system or package comprises:
- a) an automated system according to embodiments 100 or 101;
- and
- b) an effective amount of an emulsifying agent and/or a cellular membrane charging agent to reduce or disaggregate said cell aggregates, if present in said fluid sample;
- and/or, a hyperosmotic saline solution between about 350 mOsm and about 1000 mOsm, optionally between about 400 mOsm and about 600 mOsm, to reduce or disaggregate said cell aggregates, if present in said fluid sample.
Dependencies are inferred from the claim text.